Original Research Paper

Biotechnology Letters

, Volume 28, Issue 23, pp 1909-1912

Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.

  • Shi-Qing HanAffiliated withCollege of Pharmacy and Life Science, Nanjing University of Technology Email author 
  • , Ping-Kai OuyangAffiliated withCollege of Pharmacy and Life Science, Nanjing University of Technology
  • , Ping WeiAffiliated withCollege of Pharmacy and Life Science, Nanjing University of Technology
  • , Yi HuAffiliated withCollege of Pharmacy and Life Science, Nanjing University of Technology

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Abstract

The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52–100% and 88–99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.

Keywords

Biotransformation Common vetch Cyanohydrin Oxynitrilase Vicia sativa L.