Article

Biotechnology Letters

, Volume 28, Issue 7, pp 497-503

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

  • Seemanti GhoshAffiliated withDepartment of Botany, Centre of Advanced Study in Cell and Chromosome Research, University of Calcutta
  • , Biswajit GhoshAffiliated withDepartment of Botany, RKMVC College
  • , Sumita JhaAffiliated withDepartment of Botany, Centre of Advanced Study in Cell and Chromosome Research, University of Calcutta Email author 

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Abstract

Root cultures of Gloriosa superba were treated with 5 mm methyl jasmonate and 125 μ m AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mm CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mm CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchicine accumulation in root cultures.

Keywords

abiotic and biotic elicitors colchicine Gloriosa superba root culture