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Purification of Xylitol from Fermented Hemicellulosic Hydrolyzate Using Liquid–Liquid Extraction and Precipitation Techniques

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Abstract

Xylitol was produced by Candida guilliermondii by fermentation of sugarcane bagasse hemicellulosic hydrolysate. Undesirable impurities were extracted from the broth using either ethyl acetate, chloroform or dichloromethane. The best results on clarification of the broth without xylitol loss were obtained with ethyl acetate. When ethanol, acetone or tetrahydrofuran were used for precipitation of impurities, only tetrahydrofuran clarified the fermented broth, but a high xylitol loss (~30%) was observed.

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Correspondence to Silvio S. Silva.

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Mussatto, S.I., Santos, J.C., Filho, W.C.R. et al. Purification of Xylitol from Fermented Hemicellulosic Hydrolyzate Using Liquid–Liquid Extraction and Precipitation Techniques . Biotechnol Lett 27, 1113–1115 (2005). https://doi.org/10.1007/s10529-005-8458-8

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  • DOI: https://doi.org/10.1007/s10529-005-8458-8

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