Biotechnology Letters

, Volume 27, Issue 6, pp 435–442

Expression of a self-processing, pathogen resistance-enhancing gene construct in Arabidopsis

  • Haiying Liang
  • Hongyu Gao
  • Charles A. Maynard
  • William A. Powell
Article

DOI: 10.1007/s10529-005-1884-9

Cite this article as:
Liang, H., Gao, H., Maynard, C.A. et al. Biotechnol Lett (2005) 27: 435. doi:10.1007/s10529-005-1884-9
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Abstract

A gene cassette, p35S-CNO, was designed to express three gene products driven by a single constitutive CaMV 35S promoter. The individual coding regions were linked in frame to produce a single polyprotein, using spacer sequences encoding a specific heptapeptide cleavage recognition site (ENLYFQS) for the nuclear-inclusion-a (NIa) proteinase of tobacco etch virus (TEV). The protein coding sequences used were: a Trichoderma harzinum endochitinase, a truncated NIa proteinase of TEV, and a wheat oxalate oxidase. When p35S-CNO construct was tested in Arabidopsis thaliana, the polyprotein was properly cleaved after translation and the products exhibited functional enzymatic activity in vivo.

Keywords

antimicrobial proteinsdisease resistancegene pyramidplant transformationself-cleaving gene

Copyright information

© Springer 2005

Authors and Affiliations

  • Haiying Liang
    • 1
  • Hongyu Gao
    • 2
  • Charles A. Maynard
    • 3
  • William A. Powell
    • 3
  1. 1.School of Forest ResourcesThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Iowa State UniversityAmesUSA
  3. 3.College of Environmental Science and ForestryState University of New YorkSyracuseUSA