Biotechnology Letters

, Volume 27, Issue 6, pp 435–442

Expression of a self-processing, pathogen resistance-enhancing gene construct in Arabidopsis

Authors

  • Haiying Liang
    • School of Forest ResourcesThe Pennsylvania State University
  • Hongyu Gao
    • Iowa State University
  • Charles A. Maynard
    • College of Environmental Science and ForestryState University of New York
    • College of Environmental Science and ForestryState University of New York
Article

DOI: 10.1007/s10529-005-1884-9

Cite this article as:
Liang, H., Gao, H., Maynard, C.A. et al. Biotechnol Lett (2005) 27: 435. doi:10.1007/s10529-005-1884-9

Abstract

A gene cassette, p35S-CNO, was designed to express three gene products driven by a single constitutive CaMV 35S promoter. The individual coding regions were linked in frame to produce a single polyprotein, using spacer sequences encoding a specific heptapeptide cleavage recognition site (ENLYFQS) for the nuclear-inclusion-a (NIa) proteinase of tobacco etch virus (TEV). The protein coding sequences used were: a Trichoderma harzinum endochitinase, a truncated NIa proteinase of TEV, and a wheat oxalate oxidase. When p35S-CNO construct was tested in Arabidopsis thaliana, the polyprotein was properly cleaved after translation and the products exhibited functional enzymatic activity in vivo.

Keywords

antimicrobial proteinsdisease resistancegene pyramidplant transformationself-cleaving gene

Copyright information

© Springer 2005