Article

Biotechnology Letters

, Volume 27, Issue 6, pp 435-442

Expression of a self-processing, pathogen resistance-enhancing gene construct in Arabidopsis

  • Haiying LiangAffiliated withSchool of Forest Resources, The Pennsylvania State University
  • , Hongyu GaoAffiliated withIowa State University
  • , Charles A. MaynardAffiliated withCollege of Environmental Science and Forestry, State University of New York
  • , William A. PowellAffiliated withCollege of Environmental Science and Forestry, State University of New York Email author 

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Abstract

A gene cassette, p35S-CNO, was designed to express three gene products driven by a single constitutive CaMV 35S promoter. The individual coding regions were linked in frame to produce a single polyprotein, using spacer sequences encoding a specific heptapeptide cleavage recognition site (ENLYFQS) for the nuclear-inclusion-a (NIa) proteinase of tobacco etch virus (TEV). The protein coding sequences used were: a Trichoderma harzinum endochitinase, a truncated NIa proteinase of TEV, and a wheat oxalate oxidase. When p35S-CNO construct was tested in Arabidopsis thaliana, the polyprotein was properly cleaved after translation and the products exhibited functional enzymatic activity in vivo.

Keywords

antimicrobial proteins disease resistance gene pyramid plant transformation self-cleaving gene