Skip to main content
SpringerLink
Log in

Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

An Erratum to this article was published on 01 July 2005

Abstract

When docosahexaenoic acid (DHA)-producing Moritella marina strain MP-1 was cultured in the medium containing 0.5 μ g cerulenin ml−1, an inhibitor for fatty acid biosynthesis, the cells grew normally, but the␣content of DHA in the total fatty acids increased from 5.9–19.4%. The DHA yield of M. marina strain MP-1 cells also increased from 4 to 13.7 mg l−1 by cerulenin treatment. The same effect of cerulenin was observed in eicosapentaenoic acid (EPA)-producing Shewanella marinintestina strain IK-1 grown in the medium containing 7.5 μg cerulenin ml−1, and the cerulenin treatment increased the EPA yield from 1.6 to 8 mg l−1. The use of cerulenin is, therefore, advantageous to increase the content of intracellular polyunsaturated fatty acids (PUFA) in particular PUFA-containing phospholipids in bacterial cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • EE Allen D Facciotti DH Bartlett (1999) ArticleTitleMonounsaturated but not polyunsaturated fatty acids are required for growth of the deep-sea bacterium Photobacterium profundum SS9 at high pressure and low temperature Appl. Environ. Microbiol. 65 1710–1720 Occurrence Handle10103272 Occurrence Handle1:CAS:528:DyaK1MXitlartbY%3D

    PubMed  CAS  Google Scholar 

  • P Bajpai PK Bajpai (1993) ArticleTitleEicosapentaenoic acid (EPA) production from microorganisms: a review J. Biotechnol. 30 161–183 Occurrence Handle10.1016/0168-1656(93)90111-Y Occurrence Handle7764033 Occurrence Handle1:CAS:528:DyaK3sXmt1Chu7c%3D

    Article  PubMed  CAS  Google Scholar 

  • EG Bligh WJ Dyer (1959) ArticleTitleA rapid method of total lipid extraction and purification Can J. Biochem. Physiol. 37 911–917 Occurrence Handle13671378 Occurrence Handle1:CAS:528:DyaG1MXhtVSgt70%3D

    PubMed  CAS  Google Scholar 

  • J Fang C Kato T Sato O Chan D McKay (2004) ArticleTitleBiosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 137 455–461 Occurrence Handle10.1016/j.cbpc.2004.01.009 Occurrence Handle15081997 Occurrence Handle1:CAS:528:DC%2BD2cXjtVKqt7c%3D

    Article  PubMed  CAS  Google Scholar 

  • H Hibino Y Tanaka (1994) ArticleTitleMolecular structure and physiological functions of phospholipid containing docosahexaenoic acid J. Jpn. Oil Chem. Soc. 43 687–697 Occurrence Handle1:CAS:528:DyaK2cXmvFGitbg%3D

    CAS  Google Scholar 

  • C Kato Y Nogi (2001) ArticleTitleCorrelation between phylogenetic structure and function: example from deep-sea Shewanella FEMS Microbiol. Lett. 35 223–230 Occurrence Handle1:CAS:528:DC%2BD3MXislGrsLY%3D

    CAS  Google Scholar 

  • LM McMurry Oethinger M SB Levy (1988) ArticleTitleTriclosan targets lipid synthesis Nature 394 531–532 Occurrence Handle10.1038/28970 Occurrence Handle1:CAS:528:DyaK1cXltlyisbo%3D

    Article  CAS  Google Scholar 

  • N Morita M Tanaka H Okuyama (2000) ArticleTitleBiosynthesis of fatty acids in the docosahexaenoic acid-producing bacterium Moritella marina strain MP-1 Biochem. Soc. Transact. 28 943–945 Occurrence Handle10.1042/BST0280943 Occurrence Handle1:CAS:528:DC%2BD3MXhsVGqu7s%3D

    Article  CAS  Google Scholar 

  • N Morita A Ueno M Tanaka S Ohgiya T Hoshino K Kawasaki I Yumoto K Ishizaki H Okuyama (1999) ArticleTitleCloning and sequencing of clustered genes involved in fatty acid biosynthesis from the docosahexaenoic acid-producing bacterium, Vibrio marinus strain MP-1 Biotechnol. Lett. 21 641–646 Occurrence Handle10.1023/A:1005554123394 Occurrence Handle1:CAS:528:DyaK1MXlsFGju78%3D

    Article  CAS  Google Scholar 

  • D Nichols J Bowman K Sanderson CM Nichols T Lewis T McMeekin PD Nichols (1999) ArticleTitleDevelopments with antarctic micoorganisms: culture collections, bioactivity screening, taxonomy, PUFA production and cold-adapted enzymes Curr. Opin. Biotechnol. 10 240–246 Occurrence Handle10.1016/S0958-1669(99)80042-1 Occurrence Handle10361072 Occurrence Handle1:CAS:528:DyaK1MXjvVSns7s%3D

    Article  PubMed  CAS  Google Scholar 

  • H Oishi T Noto H Sasaki K Suzuki T Hayashi H Okazaki K Ando M Sawada (1982) ArticleTitleThiolactomycin, a new antibiotic. I. Taxonomy of the producing organism, fermentation and biological properties J. Antibiot (Tokyo). 35 391–395 Occurrence Handle1:CAS:528:DyaL38Xkt1ajsbw%3D

    CAS  Google Scholar 

  • C Ratledge (2004) ArticleTitleFatty acid biosynthesis in microorganisms being used for single cell oil production Biochimie 86 807–815 Occurrence Handle10.1016/j.biochi.2004.09.017 Occurrence Handle15589690 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKksLbK

    Article  PubMed  CAS  Google Scholar 

  • NJ Russell DS Nichols (1999) ArticleTitlePolyunsaturated fatty acids in marine bacteria – a dogma rewritten Microbiology 145 767–779 Occurrence Handle10220156 Occurrence Handle1:CAS:528:DyaK1MXisFWrsb4%3D Occurrence Handle10.1099/13500872-145-4-767

    Article  PubMed  CAS  Google Scholar 

  • M Satomi H Oikawa Y Yano (2003) ArticleTitleShewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic acid-producing marine bacteria isolated from sea-animal intestines Int. J. Syst. Evol. Microbiol. 53 491–499 Occurrence Handle10.1099/ijs.0.02392-0 Occurrence Handle12710618 Occurrence Handle1:CAS:528:DC%2BD3sXjtVKku78%3D

    Article  PubMed  CAS  Google Scholar 

  • A Singh OP Ward (1997) ArticleTitleMicrobial production of docosahexaenoic acid (DHA, C22:6) Adv. Appl. Microbiol. 45 271–312 Occurrence Handle9342829 Occurrence Handle10.1016/S0065-2164(08)70266-1 Occurrence Handle1:CAS:528:DyaK1cXktFSi

    Article  PubMed  CAS  Google Scholar 

  • M Tanaka A Ueno K Kawasaki I Yumoto S Ohgiya T Hoshino K Ishizaki H Okuyama N Morita (1999) ArticleTitleIsolation of clustered genes that are notably homologous to the eicosapentaenoic acid biosynthesis gene cluster from the docosahexaenoic acid-producing bacterium Vibrio marinus strain MP-1 Biotechnol. Lett. 21 939–945 Occurrence Handle10.1023/A:1005601606929 Occurrence Handle1:CAS:528:DyaK1MXotFSitLw%3D

    Article  CAS  Google Scholar 

  • Y Tanaka Y Soda S Ando (1993) ArticleTitleModulation by phospholipids of acetylcholine release from mouse brain synaptosome Neurochem. Res. 18 847

    Google Scholar 

  • H Urakawa K Kita-Tsukamoto SE Steven K Ohwada RR Colwell (1998) ArticleTitleA proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov FEMS Microbiol. Lett. 165 373–378 Occurrence Handle10.1016/S0378-1097(98)00299-7 Occurrence Handle9742712 Occurrence Handle1:CAS:528:DyaK1cXlvVSqtbg%3D

    Article  PubMed  CAS  Google Scholar 

  • RC Valentine DL Valentine (2004) ArticleTitleOmega-3 fatty acids in cellular membranes: a unified concept Prog. Lipid Res. 43 383–402 Occurrence Handle10.1016/j.plipres.2004.05.004 Occurrence Handle15458813 Occurrence Handle1:CAS:528:DC%2BD2cXotF2hu70%3D

    Article  PubMed  CAS  Google Scholar 

  • K Yazawa Y Masuzawa (1991) ArticleTitlePhospholipids – Physiological activity of phospholipids J. Jpn. Oil Chem. Soc. 40 845–857 Occurrence Handle1:CAS:528:DyaK3MXmsFCrsr8%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo, 062-8517, Japan

    Naoki Morita

  2. Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan

    Takanori Nishida, Mika Tanaka & Hidetoshi Okuyama

  3. National Research Institute of Fisheries Science, Kanazawa-ku, Yokohama, Kanagawa, 236-8648, Japan

    Yutaka Yano

Authors
  1. Naoki Morita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takanori Nishida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mika Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yutaka Yano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hidetoshi Okuyama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Naoki Morita.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-005-1633-0.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morita, N., Nishida, T., Tanaka, M. et al. Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria. Biotechnol Lett 27, 389–393 (2005). https://doi.org/10.1007/s10529-005-1532-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-1532-4

Keywords

Search

Navigation