Abstract
Because alternative RNA splicing regulation in the testis is prevalent, we explored testes of Sprague–Dawley rats for existence of alternatively spliced colony-stimulating factor 1 receptor (CSF-1R) mRNA. Using RT-PCR and sequencing, we identified a variant of CSF-1R mRNA that was 284 bp shorter than the full-length CSF-1R transcript. This variant was present in the testis (late fetal stage to adult) and in other organs of rats (7 and 60 days old). The deletion of 284 bp disrupted the open reading frame, resulting in a noncoding mRNA product. When testicular macrophages were stimulated with CSF-1R ligand and lipopolysaccharide, proportionally increased expression of both short isoform and full-length CSF-1R mRNA was observed. Thus, the identified isoform of CSF-1R mRNA may interfere with the expression of full-length CSF-1R mRNA, thereby affecting the biological activity of the ligand/receptor signaling axis in Sprague–Dawley rats.
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Acknowledgments
We thank Yvonne Löfgren Blomquist for technical support. This study was supported financially by the Stiftelsen Anna-Brita och Bo Castegrens Minne, the Stiftelsen Samariten, and Sällskapet Barnvård. Hong Su was financially supported by Stiftelsen för Barnendokrinologisk Forskning och Utbildning. Mi Hou received a grant from Svenska Sällskapet för Medicinsk Forskning (SSMF), and Olle Söder was funded by the Swedish Research Council/Finish Academy (VR-FA), the Swedish Research Council (VR), and the Pediatric Cancer Fund (BCF) in Sweden.
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Su, H., Wang, Y., Söder, O. et al. Identification of an Isoform of Colony-Stimulating Factor 1 Receptor mRNA in the Rat Testis. Biochem Genet 52, 310–319 (2014). https://doi.org/10.1007/s10528-014-9649-6
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DOI: https://doi.org/10.1007/s10528-014-9649-6