Abstract
To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2–6 alleles. The mean number of alleles per locus was 3.21. The effective number of alleles varied from 1.14 to 3.94. The observed heterozygosity varied from 0.133 to 1.00, with a mean of 0.748. The F IS values were mostly negative, with an average of −0.345. The mean F ST value was 0.056. The Nm values ranged from 1.91 to 42.17. The highest genetic identity was observed between IWI × IWK and IWK × IWI. The relation between any two groups of crosses was more than 85%. The results suggest that the crossbred populations were very closely related.
Similar content being viewed by others
References
Bhattacharya TK, Rani S, Maiti SK, Dayal S, Kumar P, Sharma A (2007) Polymorphism of ZuBeCa3 microsatellite and its association with mammary tumor in dogs. Int J Immunogenet 33:161–165
Cheng HH, Levin I, Vallejo RL, Khatib H, Dodgson JB, Crittenden LB, Hillel J (1995) Development of a genetic map of the chicken with markers of high utility. Poult Sci 74:1855–1874
Crooijmans RPMA, Groen AF, Van Kampen AJA, van der Beek S, van der Poel JJ, Groenen MAM (1996) Microsatellite polymorphism in commercial broiler and layer lines estimated using pooled blood samples. Poult Sci 75:904–909
Flock D, Preisinger R (2002) Breeding plan for poultry with emphasis on sustainability. In: Seventh World Conference on Genetic Applications for Livestock Production, 19–23 August, Montpelliers, France
Hillel J, Groenen AM, Biochard MT, Korol AB, David L, Kirzhner VM, Burke T, Dirie AB, Crooijmans RPMA, Elo K, Feldman MW, Freidlin PJ, Tanila AM, Oortwijn M, Thomson P, Vignal A, Wimmers K, Weigend S (2003) Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools. Genet Sel Evol 35:533–557
Nei M (1972) Genetic distance between populations. Am Nat 106:283–292
Nevo E (1978) Genetic variation in natural populations: patterns and theory. Theor Popul Biol 13:21–177
Olowofeso O, Wang JY, Dail GJ, Yang Y, Mekki DM, Musa HH (2005) Measurement of genetic parameters within and between Haimen chicken populations using microsatellite markers. Int J Poult Sci 4:143–148
Osman SA, Sekino M, Nishibori M, Kawamoto Y, Kinoshita K, Yamamoto Y, Tsudzuki M (2004) Genetic variability and relationships of Japanese native chickens assessed by means of microsatellite profiling approach, focussing on the Oh-Shamo (Japanese large game) and its related breeds. J Poult Sci 41:94–109
Pandey AK, Kumar Dinesh, Sharma R, Sharma U, Vijh RK, Ahlawat SPS (2005) Population structure and genetic bottleneck analysis of Ankleshwar poultry breed by microsatellite markers. Asian-Aust J Anim Sci 18:915–921
Pirany N, Romanov MN, Ganpule SP, Devegowda G, Prasad DT (2007) Microsatellite analysis of genetic diversity in Indian chicken populations. J Poult Sci 44:19–28
Rajkumar U, Gupta BR, Reddy AR (2008) Genomic heterogeneity of chicken populations in India. Asian, Aust J Anim Sci
Romanov MN, Weigend S (2001) Analysis of genetic relationships between various populations of domestic and Red Jungle fowl using microsatellite markers. Poult Sci 80:1057–1063
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, vol 1, 3rd edn. Cold Spring Harbor Laboratory Press, New York
Takahashi H, Nakamura A (2007) Genetic diversity and differentiation of the Nagoya breed inferred from microsatellite DNA polymorphisms. J Poult Sci 44:135–140
Wimmers K, Ponsuksili S, Hardge T, Valle-Zarate A, Mathur PK, Horst P (2000) Genetic distances of African, Asian and South American local chickens. Anim Genet 31:159–165
Yu YB, Wang JY, Mekki DM, Tang QP, Li HF (2006) Evaluation of genetic diversity and genetic distance between twelve Chinese indigenous chicken breeds based on microsatellite markers. Int J Poult Sci 5:550–556
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chatterjee, R.N., Bhattacharya, T.K., Dange, M. et al. Assessment of Genetic Relatedness of Crossbred Chicken Populations Using Microsatellite Markers. Biochem Genet 48, 727–736 (2010). https://doi.org/10.1007/s10528-010-9355-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-010-9355-y