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RAPD Analysis of DNA Polymorphism in Turkish Sheep Breeds

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Abstract

The genetic diversity and phylogenetic relationships of 108 individual sheep from three Turkish sheep breeds (Kıvırcık, Gökçeada, and Sakız) were studied using RAPD analysis. Polymorphisms within and between populations were assayed using 15 random primers, and 82 loci were amplified ranging from 250 to 2,500 bp. The percentage of polymorphic loci was found to be 80.49, 78.05, and 73.17%, for Kıvırcık, Gökçeada, and Sakız sheep breeds, respectively. Total genetic diversity was 0.2265, and the average coefficient of genetic differentiation was 0.1181. Genetically, the Gökçeada breed was more closely related to the Sakız breed than to the Kıvırcık breed.

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Acknowledgments

This work was supported by grants from the Scientific Research Projects Council of Uludag University (Project no. 2002–74), Bursa, Turkey.

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  1. Animal Science Department, Faculty of Agriculture, Uludag University, 16059, Bursa, Turkey

    Cengiz Elmaci, Yasemin Oner, Seniz Ozis & Erdogan Tuncel

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  1. Cengiz Elmaci
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  2. Yasemin Oner
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  3. Seniz Ozis
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  4. Erdogan Tuncel
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Correspondence to Cengiz Elmaci.

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Elmaci, C., Oner, Y., Ozis, S. et al. RAPD Analysis of DNA Polymorphism in Turkish Sheep Breeds. Biochem Genet 45, 691–696 (2007). https://doi.org/10.1007/s10528-007-9106-x

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  • DOI: https://doi.org/10.1007/s10528-007-9106-x

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