Abstract
Seven isolates of Lecanicillium lecanii (Zimmermann) Zare & Gams isolated in Spain from infected aphids were characterized using sequences of the Internal Transcribed Spacer (ITS) regions and also based on morphological and physiological characteristics. Four of these seven L. lecanii isolates were selected to assess their virulence against nymphs of Myzus persicae (Sulzer), Nasonovia ribisnigri (Mosley), Macrosiphum euphorbiae (Thomas) and Aphis gossypii Glover. Mortality (%), lethal concentration 50 (LC50) and lethal time 50 (TC50) were calculated. The analysis of the sequences of ITS region confirmed that the new isolates were clearly Lecanicillium lecanii. The set of isolates had similar radial growth (51.5–54.0 mm), except for ICAL1 (39 mm). The germination time 50 (GT50) varied between 10.7 h (ICAL3) and 13 h (ICAL5). The isolate ICAL6 showed the highest value for conidial production (3.4 × 108 con ml−1) and also produced the highest mortality for M. persicae (95%) and was more virulent than the commercial product Vertalec® (91.6%).
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Acknowledgments
We are indebted to Laura Barrios for statistical assistance, Francisco Arenal for identification assistance and to María del Mar de la Torre, María Plaza and Marina López for technical assistance. We are grateful to Koppert Biological Systems for providing us with Vertalec®. This work was funded by the Spanish Ministry of Science and Education (Research Grants AGL-2005-01449/AGR).
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Diaz, B.M., Oggerin, M., Lastra, C.C.L. et al. Characterization and virulence of Lecanicillium lecanii against different aphid species. BioControl 54, 825–835 (2009). https://doi.org/10.1007/s10526-009-9218-9
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DOI: https://doi.org/10.1007/s10526-009-9218-9