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Control of plant-parasitic nematodes by Paecilomyces lilacinus and Monacrosporium lysipagum in pot trials

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Abstract

The common soil inhabiting nematophagous fungus Paecilomyces lilacinus (Thom) Samson and the nematode trapping fungus Monacrosporium lysipagum (Drechsler) Subram were assayed for their ability to reduce the populations of three economically important plant-parasitic nematodes in pot trials. The fungi were tested individually and in combination against the root-knot nematode Meloidogyne javanica (Treub) Chitwood, cereal cyst nematode Heterodera avenae Wollenweber, or burrowing nematode Radopholus similis (Cobb) Thorne on tomato, barley and tissue cultured banana plants, respectively. In all cases, nematode populations were controlled substantially by both individual and combined applications of the fungi. Combined application of P. lilacinus and M. lysipagum reduced 62% of galls and 94% of M.␣javanica juveniles on tomato when compared to the experiment with no fungi added. Sixty five percent of H. avenae cysts were reduced on barley by combined application of fungi. Control of R. similis on banana, both in the roots and in the soil, was greatest when M. lysipagum was applied alone (86%) or in combination with P. lilacinus (96%), using a strategy where the fungi were inoculated twice in 18 weeks growth period. Overall, combined application of P. lilacinus and M. lysipagum was the most effective treatment in controlling nematode populations, although in some cases M. lysipagum alone was as effective as the combined application of fungi, particularly against M. javanica.

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Abbreviations

ANOVA:

analysis of variance

CRD:

completely randomized design

DNMRT:

Duncan’s New Multiple Range Test

PCA:

potato carrot agar

PDA:

potato dextrose agar

References

  • Al-Hazmi A.S., Ibrahim A.A.M. and Abdul-Razig A.T. (1994). Occurrence, morphology and reproduction of Heterodera avenae on wheat and barley in Saudi Arabia. Pak. J. Nematol. 12: 117–129

    Google Scholar 

  • Brand D., Roussos S., Pandey A., Zilioli P.C., Pohl J. and Soccol C.R. (2004). Development of a bionematicide with Paecilomyces lilacinus to control Meloidogyne incognita. Appl. Biochem. Biotechnol. 118: 81–88

    Article  PubMed  CAS  Google Scholar 

  • Cabanillas E. and Barker K.R. (1989). Impact of Paecilomyces lilacinus inoculum level and application time on control of Meloidogyne incognita on tomato. J. Nematol. 21: 115–120

    CAS  PubMed  Google Scholar 

  • Chen S.Y. and Dickson D.W. (1996). Fungal penetration of the cyst wall of Heterodera glycines. Phytopathology 86: 319–327

    Google Scholar 

  • Dalla-Pria M. and Ferraz S. (1996). Biological control of Meloidogyne incognita, race 3, by six species of the fungus Monacrosporium spp. alone or in combination with Verticillium chlamydosporium. Fitopatol. Brasil 21: 30–34

    Google Scholar 

  • Davide R.G. and Zorilla R.A. (1985). Evaluation of a fungus Paecilomyces lilacinus for the biological control of root-knot nematodes Meloidogyne incognita on okra as compared with nematicide Isazofos. Philipp. Agricul. 68: 493–500

    Google Scholar 

  • Dube B. (1987). Biological control of Meloidogyne incognita by Paecilomyces lilacinus and Pasteuria penetrans. J. Nematol. 19: 222–227

    CAS  PubMed  Google Scholar 

  • Eisenback J.D. and Triantaphyllou H.H. (1991). Root-knot nematodes: Meloidogyne species and races. In: Nickle, W.R. (eds) Manual of Agricultural Nematology, pp 191–274. Marcel Dekker Inc., New York

    Google Scholar 

  • Fallas G. and Sarah J.L. (1994). Effect of storage temperature on the in vitro reproduction of Radopholus similis. Nematropica 24: 175–177

    Google Scholar 

  • Gowen S. and Quénéhervé P. (1990). Nematode parasites of bananas, plantains and abaca. In: Luc, M., Sikora, R.A. and Bridge, J. (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, pp 431–460. CAB International, Wallingford, UK

    Google Scholar 

  • Hewlett T.E., Dickson D.W., Mitchell D.J. and Kannwischer-Mitchell M.E. (1988). Evaluation of Paecilomyces lilacinus as a biocontrol agent of Meloidogyne javanica on tobacco. J. Nematol. 20: 578–584

    CAS  PubMed  Google Scholar 

  • Holland, R.J., 2000. Paecilomyces lilacinus as a biocontrol agent. Doctoral Thesis, Macquarie University, Sydney, Australia

  • Holland R.J. and Williams K.L. (1996). A new technique for obtaining eggs of known age from excised females of Meloidogyne javanica. Nematologica 42: 499–502

    Article  Google Scholar 

  • Holland R.J., Williams K.L and Khan A. (1999). Infection of Meloidogyne javanica by Paecilomyces lilacinus. Nematology 1: 131–139

    Article  Google Scholar 

  • Hooper D.J. (1990). Extraction and processing of plant soil nematodes. In: Luc, M., Sikora, R.A. and Bridge, J. (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, pp 45–68. CAB International, Wallingford, UK

    Google Scholar 

  • Israeli Y., Lahav E. and Reuveni O. (1995). In vitro culture of banana. In: Gowen, S. (eds) Bananas and Plantains, pp 147–178. Chapman Hall, UK

    Google Scholar 

  • Jaffee B.A. and Muldoon A.E. (1995a). Susceptibility of root-knot and cyst nematodes to the nematode-trapping fungi Monacrosporium ellipsosporum and M. cionopagum. Soil Biol. Biochem. 27: 1083–1090

    Article  CAS  Google Scholar 

  • Jaffee B.A. and Muldoon A.E. (1995b). Numerical responses of the nematophagous fungi Hirsutella rhossiliensis, Monacrosporium cionopagum and M. ellipsosporum. Mycologia 87: 643–650

    Google Scholar 

  • Jatala P., Kaltenback R., Bocangel M., Devaus A.J. and Campos R. (1980). Field application of Paecilomyces lilacinus for controlling Meloidogyne incognita on potatoes. J. Nematol. 12: 226–227

    Google Scholar 

  • Jonathan E.I. and Rajendran G. (2000). Biocontrol potential of the parasitic fungus Paecilomyces lilacinus against the root-knot nematode Meloidogyne incognita in banana. J. Biol. Cont. 14: 67–69

    Google Scholar 

  • Kiewnick S., Lueth P. and Sikora R.A. (2002). Development of a biocontrol product based on Paecilomyces lilacinus (strain 251). Phytopathology 92: S41–S42

    Google Scholar 

  • Khan M.M.A., Mian I.H. and Zahid M.I. (1995). Post-penetration development of Meloidogyne graminicola in rice root. Thai J. Agric. Sci. 28: 311–319

    Google Scholar 

  • Kahn, A., K.L. Williams and H.K.M. Nevalainen, 2006. Infection of plant-parasitic nematodes by Paecilomyces lilacinus and Monacrosporium lysipagum. BioControl, this issue, DOI 10.1007/s10526-005-4242-x

  • Lara Martez J., Acosta N., Betancourt C., Vicente N. and Rodriguez R. (1996). Biological control of Meloidogyne incognita in tomato in Puerto Rico. Nematropica 26: 143–152

    Google Scholar 

  • Loof P.A.A. (1991). The family Pratylenchidae. In: Nickle, W.R. (eds) Manual of Agricultural Nematology, pp 363–421. Marcel Dekker Inc., New York

    Google Scholar 

  • Mankau R. and Wu X. (1985). Effects of the nematode trapping fungus, Monacrosporium ellipsosporium on Meloidogyne incognita populations in field soil. Rev. Nematol. 8: 147–153

    Google Scholar 

  • Nagesh M. and Reddy P.P. (1997). Comparative efficacy of Paecilomyces lilacinus and Verticillium lecanii in combination with botanicals against Meloidogyne incognita infecting Crossandra undulaefolia L. J. Biol. Cont. 9: 109–112

    Google Scholar 

  • Netscher C. and Sikora R.A. (1990). Nematode parasites of vegetables. In: Luc, M., Sikora, R.A. and Bridge, J. (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, pp 237–283. CAB International, Wallingford, UK

    Google Scholar 

  • Nigh E.A. and Thomson I.J. (1980). Identification and distribution of fungal parasites of Heterodera schachtii eggs in California. Phytopathology 70: 884–889

    Article  Google Scholar 

  • Ribeiro R.C.F. and Ferraz S. (2000). Evaluation of Monacrosporium spp. on the control of Meloidogyne javanica in tomato plants. Summa Phytopathologica 26: 62–68

    Google Scholar 

  • Rubner A. (1996). Revision of predacious hyphomycetes in the Dactylaria–Monacrosporium complex. Stud. Mycol. 39: 1–134

    Google Scholar 

  • Santos M.A.D., Ferraz S. and Muchovej J.J. (1992). Evaluation of 20 species of fungi from Brazil for biocontrol of Meloidogyne incognita race 3. Nematropica 22: 183–192

    Google Scholar 

  • Sasser J.N. and Freckman D.W. (1987). World perspective on nematology. The role of the society. In: Veech, J.A. and Dickson, D.W. (eds) Vistas on Nematology: A Commemoration of the 25th Anniversary of the Society of Nematologists, pp 7–14. Society of Nematologists, Inc., Hayattsville, MD

    Google Scholar 

  • Sosamma V.K., Geetha S.M. and Koshy P.K. (1994). Effect of the fungus, Paecilomyces lilacinus on the burrowing nematode, Radopholus similis infesting betel vine. Ind. J. Nematol. 24: 50–53

    Google Scholar 

  • Stemerding S. (1964). Een mixer-wattenfilter methode om vribeweeglijke endoparasitaire nematodes uit wortles te verzamelen. Verslagen Plantenziektenkundigen Dienst 141: 170–175

    Google Scholar 

  • Swarup G. and Sosa-Moss C. (1990). Nematode parasites of cereals. In: Luc, M., Sikora, R.A. and Bridge, J. (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, pp 109–136. CAB International, Wallingford, UK

    Google Scholar 

  • Villanueva L.M. and Davide R.G. (1984). Evaluation of several isolates of soil fungi for biological control of root-knot nematodes. Philipp. Agricul. 67: 361–371

    Google Scholar 

  • Wollenweber H.W. (1924). Zur Kenntnis der Kartoffel-Heteroderen. Illustrierte Landwirtschaftliche Zeitung 44: 100–101

    Google Scholar 

  • Zaki M.J. and Maqbool M.A. (1991). Combined efficacy of Pasteuria penetrans and other biocontrol agents on the control of root-knot nematode on okra. Pak. J. Nematol. 9: 49–52

    Google Scholar 

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Authors and Affiliations

  1. Proteome Systems Ltd., 1/35-41 Waterloo Road, North Ryde, NSW, 2113, Australia

    Alamgir Khan & Keith L. Williams

  2. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, 2109, Australia

    Helena K. M. Nevalainen

  3. Macquarie University Biotechnology Institute, Macquarie University, Sydney, NSW, 2109, Australia

    Helena K. M. Nevalainen

  4. Australian Proteome Analysis Facility, Macquarie University, Level 4, Building F7B, Research Park Drive, Sydney, NSW, 2109, Australia

    Alamgir Khan

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  1. Alamgir Khan
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  2. Keith L. Williams
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  3. Helena K. M. Nevalainen
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Correspondence to Alamgir Khan.

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Khan, A., Williams, K.L. & Nevalainen, H.K.M. Control of plant-parasitic nematodes by Paecilomyces lilacinus and Monacrosporium lysipagum in pot trials. Biocontrol 51, 643–658 (2006). https://doi.org/10.1007/s10526-005-4241-y

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