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Elderly mouse skeletal muscle fibres have a diminished capacity to upregulate NCAM production in response to denervation

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Abstract

Sarcopenia is a major contributor to the loss of independence and deteriorating quality of life in elderly individuals, it manifests as a decline in skeletal muscle mass and strength beyond the age of 65. Muscle fibre atrophy is a major contributor to sarcopenia and the most severely atrophic fibres are commonly found in elderly muscles to have permanently lost their motor nerve input. By contrast with elderly fibres, when fibres in young animals lose their motor input they normally mount a response to induce restoration of nerve contact, and this is mediated in part by upregulated expression of the nerve cell adhesion molecule (NCAM). Therefore, skeletal muscles appear to progressively lose their ability to become reinnervated, and here we have investigated whether this decline occurs via loss of the muscle’s ability to upregulate NCAM in response to denervation. We performed partial denervation (by peripheral nerve crush) of the extensor digitorum longus muscle of the lower limb in both young and elderly mice. We used immunohistochemistry to compare relative NCAM levels at denervated and control innervated muscle fibres, focused on measurements at neuromuscular junctional, extra-junctional and cytoplasmic locations. Muscle fibres in young animals responded to denervation with significant (32.9 %) increases in unpolysialylated NCAM at extra-junctional locations, but with no change in polysialylated NCAM. The same partial denervation protocol applied to elderly animals resulted in no significant change in either polysialylated or unpolysialylated NCAM at junctional, extra-junctional or cytoplasmic locations, therefore muscle fibres in young mice upregulated NCAM in response to denervation but fibres in elderly mice failed to do so. Elevation of NCAM levels is likely to be an important component of the muscle fibre’s ability to attract or reattract a neural input, so we conclude that the presence of increasing numbers of long-term denervated fibres in elderly muscles is due, at least in part, to the fibre’s declining ability to mount a normal response to loss of motor input.

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Acknowledgments

This work was supported by the Department of Physiology at the University of Otago. AG was the recipient of a Department of Physiology Master’s Scholarship and Postgraduate Publishing Bursary. We thank Dr Jon Cornwall, Navneet Lal, Kathrine Nielsen and Johnranin Brady for their thoughtful contributions.

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  1. Department of Physiology, Otago School of Medical Sciences, University of Otago, P.O. Box 913, Dunedin, New Zealand

    Ashley Gillon & Philip Sheard

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  1. Ashley Gillon
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  2. Philip Sheard
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Correspondence to Philip Sheard.

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Gillon, A., Sheard, P. Elderly mouse skeletal muscle fibres have a diminished capacity to upregulate NCAM production in response to denervation. Biogerontology 16, 811–823 (2015). https://doi.org/10.1007/s10522-015-9608-6

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