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Morphofunctional Characteristics of Human Lymphocytes after In Vitro Activation

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Bulletin of Experimental Biology and Medicine Aims and scope

We describe a method of activation of lymphocytes isolated from the peripheral blood of patients with cutaneous melanoma and cultured in serum-free medium in the presence of IL-2 and IL-15. Viability, proliferative, cytotoxic, and functional activities of lymphocytes are evaluated. The lymphocytes actively proliferated in this nutrient medium and can be activated in vitro. The method for obtaining sufficient amount of activated lymphocytes can be recommended for adoptive cell immunotherapy of cancer patients.

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Authors and Affiliations

  1. A. Tsyb Medical Radiological Research Centre – Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation, Obninsk, Russia

    E. V. Abakushina, Yu. V. Marizina & A. D. Kaprin

Authors
  1. E. V. Abakushina
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  2. Yu. V. Marizina
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  3. A. D. Kaprin
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Correspondence to E. V. Abakushina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 5, pp. 678-683, May, 2016

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Abakushina, E.V., Marizina, Y.V. & Kaprin, A.D. Morphofunctional Characteristics of Human Lymphocytes after In Vitro Activation. Bull Exp Biol Med 161, 731–735 (2016). https://doi.org/10.1007/s10517-016-3496-x

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  • DOI: https://doi.org/10.1007/s10517-016-3496-x

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