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Biological Effects of Individually Synthesized TNF-Binding Domain of Variola Virus CrmB Protein

  • Virology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the effi ciency of its inhibition of TNF cytotoxicity towards L929 mouse fi broblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.

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Authors and Affiliations

  1. Research Institute of Clinical Immunology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia

    D. D. Tsyrendorzhiev, I. A. Orlovskaya, S. V. Sennikov & M. D. Tsyrendorzhieva

  2. Novosibirsk State Medical University, Ministry of Health and Social Development of the Russian Federation, Novosibirsk, Russia

    D. D. Tsyrendorzhiev & M. D. Tsyrendorzhieva

  3. Vector State Research Center, Kol’tsovo, Novosibirsk Region, Novosibirsk, Russia

    T. V. Tregubchak, I. P. Gileva & S. N. Shchelkunov

  4. Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    S. N. Shchelkunov

Authors
  1. D. D. Tsyrendorzhiev
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  2. I. A. Orlovskaya
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  3. S. V. Sennikov
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  4. T. V. Tregubchak
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  5. I. P. Gileva
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  6. M. D. Tsyrendorzhieva
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  7. S. N. Shchelkunov
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Correspondence to D. D. Tsyrendorzhiev.

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Tsyrendorzhiev, D.D., Orlovskaya, I.A., Sennikov, S.V. et al. Biological Effects of Individually Synthesized TNF-Binding Domain of Variola Virus CrmB Protein. Bull Exp Biol Med 157, 249–252 (2014). https://doi.org/10.1007/s10517-014-2537-6

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  • DOI: https://doi.org/10.1007/s10517-014-2537-6

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