The content of ATP in scalp hair bulbs in humans was measured in the hair roots from 15 healthy volunteers. Light and electron microscopy confirmed the presence of outer and an inner root sheaths in the root of pulled out anagen hair. Incubation of samples in buffer solution led to extraction of ATP, which was measured by the chemiluminescent method. Mechanic disintegration of hair bulbs and their freezing–defrosting did not increase ATP output. The results of microscopy indicated that ATP extraction procedure was associated with separation of the outer radical sheath from the inner one without impairing the structure of the inner sheath. The mean content of ATP was 12±2 pmol per bulb. The use of pulled out hair bulbs for ATP measurements simplified the procedure as involved no surgical removal of follicles.
Similar content being viewed by others
References
K. Adachi, S. Takayasu, I. Takashima, et al., J. Soc. Cosmet. Chem., 21, No. 13, 901-924 (1970).
C. Cipriani, G. Moretti, E. Rampini, and C. Divano, Arch. Derm. Res., 256, No. 3, 319-325 (1976).
G. Gazzani, S. Roncoroni, M. Corsi, et al., Int. J. Tissue React., 16, No. 2, 73-77 (1994).
A. M. Glauert, Fixation, Dehydration, and Embedding of Biological Specimens, Amsterdam (1975), P. 207.
A. V. Greig, C. Linge, and G. Burnstock, Purinergic Signal, 4, No. 4, 331-338 (2008).
M. Ibraheem, H. Galbraith, J. Scalife, and S. Ewen, J. Anat., 182, Pt. 2, 231-238 (1993).
E. S. Reynolds, J. Cell. Biol., 17, No. 1, 208-212 (1963).
G. E. Rogers, Int. J. Dev. Biol., 48, Nos. 2-3, 163-170 (2004).
K. Shorter, N. Farjo, S. M. Picksley, and V. A. Randall, FASEB J., 22, No. 6, 1725-1736 (2008).
R. Williams, M. P. Philpott, and T. Kealey, J. Invest. Dermatol., 100, No. 6, 834-840 (1993).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 157, No. 1, pp. 125-128, January, 2014
Rights and permissions
About this article
Cite this article
Mikhal’chik, E.V., Suprun, M.V., Fedorkova, M.V. et al. Evaluation of ATP Content in Hair Bulbs in Human Scalp. Bull Exp Biol Med 157, 112–115 (2014). https://doi.org/10.1007/s10517-014-2504-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-014-2504-2