The effects of water-dispersed Ag nanoparticles on the small intestinal mucosa, liver, and spleen of rats were studied by transmission electron microscopy. Acute experiments demonstrated penetration of Ag nanoparticles injected into the isolated intestinal loop into the intestinal mucosa, liver, and splenic tissues. Ultrastructural changes (lobed nucleus, megamitochondria) were found in the studied organs. These data indicated that injection of waterdispersed Ag nanoparticles into the gastrointestinal tract was followed by their penetration through the epithelium of the small intestinal mucosa into other organs, e.g. into the liver and spleen. This fact is essential for evaluation of potential risks of the nanoparticle effects on human health and environment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. P. Avtsyn and V. A. Shakhlamov, Ultrastructural Bases of Cell Pathology [in Russian], Moscow (1979).
N. N. Bezborodkina, S. V. Okovityi, M. V. Kudryavtseva, et al., Tsitologiya, 50, No. 3, 228–236 (2008).
V. M. Vernikov, E. A. Arianova, I. V. Gmoshinski, et al., Vopr. Pitan., 78, No. 2, 4–17 (2009).
V. M. Vernikov, I. V. Gmoshinski, and S. A. Khotimchenko, Ibid., 78, No. 6, 13–20 (2009).
A. A. Mironov, Ya. Yu. Komisarchuk, and V. A. Mironov, Me thods of Electron Microscopy and Biology. Methodology Manual [in Russian], St. Petersburg (1994).
I. A. Morozov, S. I. Khvylya, and Yu. A. Lysikov, Fiziol. Zh. SSSR, 68, No. 9, 1261–1267 (1982).
V. V. Nevzorova, I. V. Gmoshinski, and S. A. Khotimchenko, Vopr. Pitan., 78, No. 4, 54–60 (2009).
K. I. Popov, I. V. Gmoshinski, A. N. Filippov, et al., Food Nanotechnologies: Prospects and Problems [in Russian], Moscow (2010).
A. I. Strukov and V. V. Serov, Morbid Anatomy {in Russian], Moscow (1993).
A. A. Shumakova, V. V. Smirnova, O. N. Tananova, et al., Vopr. Pitan., 80, No. 6, 9–18 (2011).
S. A. Blaser, M. Scheringer, M. Macleod, and K. Hungerbuhler, Sci. Total Environ., 390, Nos. 2-3, 396–409 (2008).
Q. Chaudhry, M. Scotter, J. Blackburn, et al., Food Additives Contam. Part A. Chem. Anal. Control Exp. Risk Assess., 25, No. 3, 241–258 (2008).
Y. S. Kim, M. Y. Song, J. D. Park, et al., Part Fibre Toxicol., 7, 20 (2010).
H. S. Sharma, S. Hussain, J. Schlager, et al., Acta Neurochir., Suppl., 106, 359–364 (2010).
K. Welt, J. Weiss, R. Martin, et al., Exp. Toxicol. Pathol, 55, No. 5, 331–345 (2004).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 155, No. 2, pp. 204-209, February, 2013
Rights and permissions
About this article
Cite this article
Platonova, T.A., Pridvorova, S.M., Zherdev, A.V. et al. Identification of Silver Nanoparticles in the Small Intestinal Mucosa, Liver, and Spleen of Rats by Transmission Electron Microscopy. Bull Exp Biol Med 155, 236–241 (2013). https://doi.org/10.1007/s10517-013-2122-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-013-2122-4