Abstract
Fucoidan mainly produced by brown algae is a sulfated polysaccharide with fucose as a main molecule backbone. The objective of this research was to evaluate the non-specific immune potentiating activity of fucoidan from a tropical brown alga, Sargassum cristaefolium indigenous to the Gunungkidul coast, Yogyakarta, Indonesia. Fucoidan was extracted by acidic extraction, separated from alginate by CaCl2 precipitation and precipitated with ethanol. To know the component of fucoidan, acid hydrolysis was conducted and analyzed by using silica gel thin layer chromatography. In these analyses, a commercial fucoidan from Fucus vesiculosus was used as a standard fucoidan. Fucoidan isolated from S. cristaefolium was composed by monosaccharide components of fucose and galactose. The result revealed that injection of fucoidan increased phagocytic activity, total plasma protein, leukocrit and leukocyte count significantly (p < 0.05). The immunomodulating activity of fucoidan from S. cristaefolium was comparable to that of fucoidan standard from F. vesiculosus. However, administration of fucoidan did not affect the phagocytic index and leukocyte differentiation. Injection of fucoidan from S. cristaefolium effectively increased innate immunity parameters of tilapia at doses of 0.4–0.6 mg/kg fish suggested that this fucoidan was potential use for immunostimulant to control fish diseases.
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References
Anderson DP (1992) Immunostimulant, adjuvant and vaccine carrier in fish, application in aquaculture. Ann Rev Fish Dis 9:2106–2130
Anderson DP, Siwicki AK (1994) Simplified assays for measuring non-specific defence mechanism in fish. Fish Health Section/American Fisheries Meeting, Seatle Washington
Anno K, Terahata H, Hayashi Y (1966) Isolation and purification of fucoidan from brown seaweed Pelvetia wrightii. Agric Biol Chem 30:495–499
Beress A, Wassermann O, Bruhn T, Beress L (1993) A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus. J Nat Prod 56:478–488
Berteau O, Mulloy B (2003) Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharides. Glycobiology 13:29R–40R
Bittencourt NLR, Molinari LM, Scoaris DO, Pedroso RB, Nakamura CV, Nakamura TU, Filho BAA, Filho BPD (2003) Haematological and biochemical values for nile tilapia Oreochromis niloticus cultured in semi-intensive system. Biol Sci 25:385–389
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Castro R, Zarra I, Lamas J (2003) Water-soluble seaweed extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture 229:67–78
Chotigeat W, Tongsupa S, Kidchakan S, Phongdara A (2004) Effect of fucoidan on disease resistance of black tiger shrimp. Aquaculture 233:23–30
de Souza MCR, Marques CT, Dore CMG, da Silva FRF, Rocha HAO, Leite EL (2007) Antioxidant activities of sulfated polysaccharides from brown and red seaweeds. J Appl Phycol 19:153–160
Doh-ura K, Kuge T, Uomoto M, Nishizawa K, Kawasaki Y, Iha M (2007) Prophylactic effect of dietary seaweed fucoidan against enteral prion infection. Antimicrob Agents Chemother 51:2274–2277
Duarte MER, Cardoso MA, Noseda MD, Cerezo AS (2001) Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum. Carbohydr Res 333:281–293
Duranda E, Helleya D, Zena AAH, Dujolsd C, Brunevala P, Colliec-Jouaultf S, Fischera A-M, Lafonta A (2008) Effect of low molecular weight fucoidan and low molecular weight heparin in a rabbit model of arterial thrombosis. J Vasc Res 45:529–537
Hayashi S, Itoh A, Isoda K, Kondoh M, Kawase M, Yagi K (2008) Fucoidan partly prevents CCl4-induced liver fibrosis. Eur J Pharmacol 580:380–384
Hoshino T, Hayashi T, Hayashi J, Lee JB, Sankawa U (1998) An antivirally active sulfated polysaccharide from Sargassum horneri (TURNER) C. AGARDH. Biol Pharmacol Bull 21:730–734
Hussein MM, Abdel A, Salem HM (1980) Some structural features of a new sulfated heteropolysaccharide from Padina pavoia. Phytochemistry 19:2133–2135
Immanuel G, Sivagnanavelmurugan M, Marudhupandi T, Radhakrishnan S, Palavesam A (2012) The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab). Fish Shellfish Immunol 32:551–564
Kawamoto H, Miki Y, Kimura T, Tanaka K, Nakagawa T, Kawamukai M, Matsuda H (2006) Effects of fucoidan from Mozuku on human stomach cell lines. Food Sci Technol Res 12:218–222
Kim W-J, Kim S-M, Kim HG, Oh H-R, Lee K-B, Lee Y-K, Park Y-I (2007) Purification and anticoagulant activity of a fucoidan from Korean Undaria pinnatifida Sporophyll. Algae 22:247–252
Kim EJ, Park SY, Lee J-Y, Park JHY (2010a) Fucoidan present in brown algae induces apoptosis of human colon cancer cells. BMC Gastroenterol 10:96
Kim WJ, Koo Y-K, Jung M-K, Moon HR, Kim SM, Synystsya A, Yun-Choi HS, Kim YS, Park JK, Parl YI (2010b) Anticoagulating activities of low-molecular Weight Fuco-Oligosaccharides prepared by enzymatic digestion of fucoidan from the sporophyll of Korean Undaria pinnatifida. Arch Pharm Res 33:125–131
Lee YK, Lim D-J, Lee Y-H, Park Y-I (2006) Variation in fucoidan contents and monosaccharide compositions of Korean Undaria pinnatifida (Harvey) Suringar (Phaeophyta). Algae 21:157–160
Li B, Lu F, Wei X, Zhao R (2008) Fucoidan: structure and bioactivity. Molecules 13:1671–1695
Makoto F, De-Xing H, Masaji N (2005) Fucoidan-based health food. USA Patent 20050129708
Mourao PAS, Pereira MS (1999) Searching for alternatives to heparin: sulfated fucans from marine invertebrates. Trends Cardiovasc Med 9:225–232
Nishino T, Nishioka C, Ura H, Nagumo T (1994) Isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial Fucus vesiculosus fucoidan. Carbohydr Res 255:213–224
Ostergaard C, Yieng-Kow RV, Benfield T, Frimodt-Moller N, Espersen F, Lundgren JD (2000) Inhibition of leukocyte entry into the brain by the selectin blocker fucoidin decreases interleukin-1 (IL-1) levels but increases IL-8 levels in cerebrospinal fluid during experimental Pneumococcal meningitis in rabbits. Infect Immununol 68:3153–3157
Peddie S, Zou J, Secombes CJ (2002) Immunostimulation in the rainbow trout (Oncorhynchus miykiss) following intraperitoneal administration of ergosan. Vet Immunol Immunophatol 86:101-113
Pielesz A, Binias W (2010) Cellulose acetate membrane electrophoresis and FTIR spectroscopy as methods of identifying a fucoidan in Fucus vesiculosus Linnaeus. Carbohydr Res 345:2676–2682
Pomin VH, Mourão PAS (2008) Structure, biology, evolution, and medical importance of sulfated fucans and galactans. Glycobiology 18:1016–1027
Ramberg JE, Nelson ED, Sinnott RA (2010) Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutr J 9:54
Sakai M (1999) Current research status of fish immunostimulants. Aquaculture 172:63–92
Sarlin PJ, Philip R (2011) Efficacy of marine yeasts and baker’s yeast as immunostimulants in Fenneropenaeus indicus: a comparative study. Aquaculture 321:173–178
Sebastião FA, Nomura D, Sakabe R, Pilarski F (2010) Hematology and productive performance of nile tilapia (Oreochromis niloticus) naturally infected with Flavobacterium columnare. Braz J Microbiol 42:282–289
Selvaraj V, Sampath K, Sekar V (2005) Administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (Cyprinus carpio) infected with Aeromonas hydrophila. Fish Shellfish Immunol 19:293–306
Silva TMA, Alves LG, de Queiroz KCS, Santos MGL, Marques CT, Chavante SF, Rocha HAO, Leite EL (2005) Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora. Braz J Med Biol Res 38:523–533
Teruya T, Takeda S, Tamaki Y, Tako M (2010) Fucoidan isolated from Laminaria angustata var longissima induced macrophage activation. Biosci Biotechnol Biochem 74:1960–1962
Wang Q, Song Y, He Y, Ren D, Kow F, Qiao Z, Liu S, Yu X (2014) Structural characterisation of algae Costaria costata fucoidan and its effects on CCl4-induced liver injury. Carbohydr Polym 107:247–254
Watanuki H, Chakraborty G, Korenaga H, Kono T, Shivappa RB, Sakai M (2009) Immunostimulatory effects of natural human interferon-alpha (huIFN-a) on carps Cyprinus carpio L. Vet Immunol Immunopathol 131:273–277
Ye J, Li Y, Teruya K, Katakura Y, Ichikawa A, Eto H, Hosoi M, Hosoi M, Nishimoto S, Shirahata S (2005) Enzyme-digested fucoidan extracts derived from seaweed Mozuku of Cladosiphon novae-caledoniae kylin inhibit invasion and angiogenesis of tumor cells. Cytotechnology 47:117–126
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Part of this research was financially supported by a grant from Directorate of Higher Education, Ministry of Education and Culture, the Republic of Indonesia, Financial Year 2013/2014.
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Isnansetyo, A., Fikriyah, A., Kasanah, N. et al. Non-specific immune potentiating activity of fucoidan from a tropical brown algae (Phaeophyceae), Sargassum cristaefolium in tilapia (Oreochromis niloticus). Aquacult Int 24, 465–477 (2016). https://doi.org/10.1007/s10499-015-9938-z
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DOI: https://doi.org/10.1007/s10499-015-9938-z