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Application of nanosilver-coated zeolite as water filter media for fungal disinfection of rainbow trout (Oncorhynchus mykiss) eggs

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Abstract

This study investigated the indirect use of silver nanoparticles (AgNPs) for reduction of fungal infections during incubation period of fertilized rainbow trout eggs. Different concentrations of nanosilver-coated zeolite (0.5, 1, and 1.5 % AgNPs) were compared with unmodified zeolite as water filter media in semi-recirculation systems. For testing the effect of AgNPs on reduction of fungal infection, fertilized eggs were transferred in incubators receiving water from filters coated with nanosilver. The eggs in each incubator were inoculated with Saprolegnia-infected trout eggs. Any dead or infected eggs and embryos were periodically removed, while the performance of the filters was assessed by calculating the survival rates from fertilization up to completion of the yolk–sac absorption stage. The results showed that the filters containing 0.5 % AgNPs increased the survival rate by 4.56 % from fertilization to the swim up stage compared to the control (p < 0.05). Also, the additional application of activated carbon (as absorbent media) along with AgNP-coated media in filters caused an increase of about 11.24 % in the survival rate for the larval stage (p < 0.05). In contrast to the control group with about 6 % fungal infection, no infections were observed during the incubation period in the incubators containing nanosilver-coated filters. Therefore, the final results confirmed that the indirect use of AgNPs in the aforementioned filters were significantly effective for preventing fungal infections in semi-recirculation systems for rainbow trout, making them a candidate for replacing the chemical fungicides currently used during egg incubation in hatchery systems.

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Acknowledgments

The authors gratefully acknowledge the support of the Tarbiat Modares University of I. R. Iran, who funded this research through a Ph.D. thesis project. This research was also supported by the Nanomaterial Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education, Science and Technology (No. 2012-006648).

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  1. Seyed Ali Johari

    Present address: Fisheries Department, Natural Resources Faculty, University of Kurdistan, ZIP Code: 66177-15175, P.O. Box 416, Sanandaj, Kurdistan, Iran

Authors and Affiliations

  1. Department of Aquaculture, Marine Science Faculty, Tarbiat Modares University, P.O. Box 46414-356, Noor, Mazandaran, Iran

    Seyed Ali Johari & Mohammad Reza Kalbassi

  2. Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Mehdi Soltani

  3. Institute of Nanoproduct Safety Research, Hoseo University, Asan, Korea

    Il Je Yu

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  1. Seyed Ali Johari
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Correspondence to Seyed Ali Johari or Mohammad Reza Kalbassi.

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Johari, S.A., Kalbassi, M.R., Soltani, M. et al. Application of nanosilver-coated zeolite as water filter media for fungal disinfection of rainbow trout (Oncorhynchus mykiss) eggs. Aquacult Int 24, 23–38 (2016). https://doi.org/10.1007/s10499-015-9906-7

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