Abstract
As the cheap and sustainable recycled natural resources, intestinal casing meal (ICM) and yeast of monosodium glutamate (YMG) might be alternative protein sources in aquaculture diets. To determine the effects of dietary ICM and YMG on fish growth, feed utilization and gut allochthonous bacteria, 8-week feeding trials were conducted with blunt snout bream (Megalobrama amblycephala, MA; a typical herbivorous cultured fish species in China), black carp (Mylopharyngodon piceus, MP; a typical carnivorous species in China) and gibel carp (Carassius gibelio, CG; a typical omnivorous species in China) with partial or complete replacement of soybean meal by ICM or ICM and YMG mixture. The results indicated that ICM and YMG incorporation had positive effects (P value >0.05) on weight gain and feed conversion rate. The complete replacement altered the gut allochthonous microbiota of gibel carp and black carp (Cs < 0.70). Proteobacteria, Fusobacteria, Firmicutes and uncultured bacteria were predominant bacteria in these fish intestine by DGGE detection. Compared with the control diet group with SBM, intestinal allochthonous bacteria of fishes fed with ICM and YMG were significantly stimulated, such as proteobacterium clone (EF707282.1), Cetobacterium somerae (AB353124) and some Bacillus subtilis dependent on fish species. Based on these results, ICM and YMG have the potential to replace SBM in aqua feed, and altered gut allochthonous bacteria variedly as fish food habitats.
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Abbreviations
- CF:
-
Condition factor
- CG:
-
Carassius gibelio
- Cs:
-
Coefficients
- DGGE:
-
16S rDNA PCR–denaturing gradient gel electrophoresis
- FBW:
-
The final body weight of fish
- FCR:
-
Feed conversion ratio
- IBW:
-
The initial body weight of fish
- ICM:
-
Intestinal casing meal
- ITd :
-
The total dry diet consumption
- MA:
-
Megalobrama amblycephala
- MP:
-
Mylopharyngodon piceus
- SBM:
-
Soybean meal
- SR:
-
Survival rate
- T :
-
The experimental days
- TI:
-
Trypsin inhibitors
- WG:
-
Weight gains
- YMG:
-
Yeast of monosodium glutamate
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This work was supported by the National Science and Technology Support Program Project of China (2012BAD25B02), the National Basic Research Program of China (2014CB138600), the National Natural Science Foundation of China (30972265), and Beijing earmarked fund for Modern Agro-industry Technology Research System (SCGWZJ 20121104-4).
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Zhimin Li and Li Xu have contributed equally to this work.
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Li, Z., Xu, L., Liu, W. et al. Protein replacement in practical diets altered gut allochthonous bacteria of cultured cyprinid species with different food habits. Aquacult Int 23, 913–928 (2015). https://doi.org/10.1007/s10499-014-9851-x
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DOI: https://doi.org/10.1007/s10499-014-9851-x