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Effects of nitrate toxicity in the Pacific white shrimp, Litopenaeus vannamei, reared with biofloc technology (BFT)

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Abstract

In cultures of the Litopenaeus vannamei with biofloc, the concentrations of nitrate rise during the culture period, which may cause a reduction in growth and mortality of the shrimps. Therefore, the aim of this study was to determine the effect of the concentration of nitrate on the growth and survival of shrimp in systems using bioflocs. The experiment consisted of four treatments with three replicates each: The concentrations of nitrate that were tested were 75 (control), 150, 300, and 600 mg NO3 −-N/L. To achieve levels above 75 mg NO3 −-N/L, different dosages of sodium nitrate (PA) were added. For this purpose, twelve experimental units with a useful volume of 45 L were stocked with 15 juvenile L. vannamei (1.30 ± 0.31 g), corresponding to a stocking density of 333 shrimps/m3, that were reared for an experimental period of 42 days. Regarding the water quality parameters measured throughout the study, no significant differences were detected (p > 0.05). Concerning zootechnical performance, a significant difference (p < 0.05) was verified with the 75 (control) and 150 treatments presenting the best performance indexes, while the 300 and 600 treatments led to significantly poorer results (p < 0.05). The histopathological damage was observed in the gills and hepatopancreas of the shrimps exposed to concentrations ≥300 mg NO3 −-N/L for 42 days, and poorer zootechnical performance and lower survival were observed in the shrimps reared at concentrations ≥300 mg NO3 −-N/L under a salinity of 23. The results obtained in this study show that concentrations of nitrate up to 177 mg/L are acceptable for the rearing of L. vannamei in systems with bioflocs, without renewal of water, at a salinity of 23.

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Acknowledgments

The authors are grateful for the financial support provided by the National Council for Scientific and Technological Development (CNPq), the Ministry of Fishery and Aquaculture (MPA), and the Coordination for the Improvement of Higher Level Personnel (CAPES). Special thanks go to Centro Oeste Rações S.A. for donating the experimental diets. W.J. Wasielesky Jr. and L.H. Poersch are research fellows at CNPq.

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  1. Marine Station of Aquaculture, Institute of Oceanography, Federal University of Rio Grande, C. P. 474, Rio Grande, RS, CEP 96201-900, Brazil

    Plínio S. Furtado, Bruno R. Campos, Fabiane P. Serra, Marta Klosterhoff, Luis A. Romano & Wilson Wasielesky Jr.

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  1. Plínio S. Furtado
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  2. Bruno R. Campos
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  3. Fabiane P. Serra
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  4. Marta Klosterhoff
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  5. Luis A. Romano
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  6. Wilson Wasielesky Jr.
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Correspondence to Wilson Wasielesky Jr..

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Furtado, P.S., Campos, B.R., Serra, F.P. et al. Effects of nitrate toxicity in the Pacific white shrimp, Litopenaeus vannamei, reared with biofloc technology (BFT). Aquacult Int 23, 315–327 (2015). https://doi.org/10.1007/s10499-014-9817-z

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