Abstract
Production of sterile triploid red tilapia [Oreochromis mossambicus (Mozambique tilapia); Peters, 1852 × Oreochromis niloticus (Nile tilapia); Linnaeus, 1758] is an effective strategy to overcome their prolific breeding. Optimal conditions for cold-shock induction of triploidy in red tilapia were investigated by experimentally examining two variables: appropriate temperature of the shock and duration of shock treatment. A constant time after insemination of 4 min was used to determine the best combination of temperature (6, 7, 8, 9, 11, 13, 15 °C) with different durations of shock (10, 20, 30, 40, 50 min) with resultant ploidy level verified karyotypically. Shock duration for 30 min at a temperature of 9 °C was found most effective in producing maximum triploidy (98.7 %) with higher rates of hatching (63.2 %) and survival up to yolk-sac stage (75.8 %). The chromosome count confirmed that triploid percentages were higher when cold shock was used for longer durations at each temperature; however, hatching rates were generally decreased. The maximum triploid yield (82.1 %) obtained was higher than the yield obtained using heat shock (72.7 %) in red tilapia previously. The application of the results of this study has the potential to greatly improve the production of triploid red tilapia in commercial aquaculture.
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Acknowledgments
The authors are grateful to the Vice Chancellor, University Malaysia, Terengganu, Malaysia, for providing laboratory facilities. The authors (PPJ and STC) are also thankful to University Malaysia, Terengganu, for the award of research assistantships, whereas the author (AC) for the award of a Principal Research Fellowship. The authors are also thankful to two anonymous reviewers for their critical comments to improve the quality of the manuscript.
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Pradeep, P.J., Srijaya, T.C., Hassan, A. et al. Optimal conditions for cold-shock induction of triploidy in red tilapia. Aquacult Int 22, 1163–1174 (2014). https://doi.org/10.1007/s10499-013-9736-4
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DOI: https://doi.org/10.1007/s10499-013-9736-4