Abstract
Molecular markers (PCR–RFLP and microsatellite) were used to investigate the genetic background of the only brown trout (Salmo trutta m fario) broodstock in Hungary which due to the hydrogeography of the country should theoretically belong to the Danubian lineage. PCR–RFLP (mitochondrial DNA control region and lactate dehydrogenase and somatolactin genes) as well as microsatellite (BFRO002, OMM1064, Ssa408uos, SsoSL417, SsoSL438) markers were used to distinguish between Danubian and Atlantic lineages of brown trout in the Lillafüred broodstock. Altogether 435 fish were tagged during the experimental period. Due to mortalities, fin clips were collected from 401 individuals. According to the genetic analysis of the mitochondrial DNA, the Danubian haplotype is present in only one individual (0.2 %) of the broodstock. Analysis of the nuclear markers revealed that alleles characteristic of both the Atlantic and the Danubian lineages are found in the population. However, Atlantic alleles dominate throughout the broodstock which is in Hardy–Weinberg equilibrium according to the investigated markers. Results indicate that the original broodstock that was introduced to the farm following its construction in 1933 was of the Atlantic lineage. Although later fish from a local stream were collected and added to the broodstock, the number of these was limited and they were almost exclusively males. Fish from this farm that are stocked by anglers can have a significant genetic impact on trout populations of natural streams.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bernatchez L (2001) The evolutionary history of brown trout (Salmo trutta L) inferred from phylogeographic, nested clade, and mismatch analyses of mitochondrial DNA variation. Evolution 55:351–379
Bernatchez L, Danzmann RG (1993) Congruence in control-region sequence and restriction-site variation in mitochondrial DNA of Brook Charr (Salvelinus fontinalis Mitchill). Mol Biol Evol 10(5):1002–1014
Bogataj K (2010) Genetic analysis of native brown trout (Salmo trutta) populations in Slovenia. Thesis, University of Ljubljana
Cairney M, Taggart JB, Høyheim B (2000) Characterization of microsatellite and minisatellite loci in Atlantic salmon (Salmo salar L.) and cross-species amplification in other salmonids. Mol Ecol 9:2175–2178
Ford MJ (1998) Testing models of migration and isolation among populations of Chinook salmon (Onchorynchus tshawytscha). Evolution 52(2):539–557
Jug T, Berrebi P, Snoj A (2005) Distribution of non-native trout in Slovenia and their introgression with native trout populations as observed through microsatellite DNA analysis. Biol Conserv 123:381–388
Marić S, Sušnik S, Simonović P, Snoj A (2006) Phylogeographic study of brown trout from Serbia, based on mitochondrial DNA control region analysis. Genet Sel Evol 38:411–430
Marić S, Simonović P, Razpet A (2010) Genetic characterization of broodstock brown trout from Bled fish-farm, Slovenia. Period Biol 112:145–148
McMeel OM, Hoey EM, Ferguson A (2001) Partial nucleotide sequences, and routine typing by polymerase chain reaction–restriction fragment length polymorphism, of the brown trout (Salmo trutta) lactate dehydrogenase, LDH-C1*90 and *100 alleles. Mol Ecol 10:29–34
Muhlfeld CC, Kalinowski ST, McMahon TE, Taper ML, Painter S, Leary RF, Allendorf FW (2009) Hybridization rapidly reduces fitness of a native trout in the wild. Biol Let 5:328–331
Rexroad CE, Coleman RL, Hershberger WK, Killefer J (2002) Rapid communication: thirty-eight polymorphic microsatellite markers for mapping in rainbow trout. J Anim Sci 80:541–542
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. CSH Laboratory Press, Cold Spring Harbor, NY
Shimizu M, Kosaka N, Shimada T, Nagahata T, Iwasaki H, Nagai H, Shiba T, Emi M (2002) Universal fluorescent labeling (UFL) method for automated microsatellite analysis. DNA Res 9:173–178
Slettan A, Olsaker I, Lie Ø (1995) Atlantic salmon, Salmo salar, microsatellites at the SSOSL25, SSOSL85, SSOSL311, SSOSL417 loci. Anim Genet 26:281–282
Slettan A, Olsaker I, Lie Ø (1996) Polymorphic Atlantic salmon, Salmo salar L., microsatellites at the SSOSL438. SSOSL439 and SSOSL444 loci. Anim Genet 27:57–58
Snoj A, Marčeta B, Sušnik S, Melkič E, Meglič V, Dovč P (2002) The taxonomic status of the ‘sea trout’ from the north Adriatic Sea, as revealed by mitochondrial and nuclear DNA analysis. J Biogeogr 29:1179–1185
Snoj A, Glamuzina B, Razpet A, Zablocki J, Bogut I, Lerceteau-Köhler E, Pojskić N, Sušnik S (2010) Resolving taxonomic uncertainties using molecular systematics: Salmo dentex and the Balkan trout community. Hydrobiologia 651:199–212
Sušnik S, Snoj A, Pohar J, Dovč P (1997) The microsatellite marker (BFRO 002) characteristic for different geographically remote brown trout, Salmo trutta L., populations. Anim Genet 28:372
Sušnik S, Snoj A, Dovč P (2001) Evolutionary distinctness of grayling (Thymallus thymallus) inhabiting the Adriatic river system, as based on mtDNA variation. Biol J Linnean Soc 74:375–385
Taylor EB (1991) A review of local adaptation in Salmonidae, with particular reference to Pacific and Atlantic salmon. Aquaculture 98:185–207
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
The work was supported by the GOP‐111‐09/1‐2010‐0141 project.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Horváth, Á., Hoitsy, G., Kovács, B. et al. The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary. Aquacult Int 22, 5–11 (2014). https://doi.org/10.1007/s10499-013-9665-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10499-013-9665-2