Apoptosis

, Volume 18, Issue 2, pp 238–247

An in vivo molecular imaging probe 18F-Annexin B1 for apoptosis detection by PET/CT: preparation and preliminary evaluation

  • Ming-Wei Wang
  • Fang Wang
  • Yu-Jia Zheng
  • Ying-Jian Zhang
  • Yong-Ping Zhang
  • Qing Zhao
  • Clifton Kwang-Fu Shen
  • Yue Wang
  • Shu-Han Sun
Original Paper

DOI: 10.1007/s10495-012-0788-0

Cite this article as:
Wang, M., Wang, F., Zheng, Y. et al. Apoptosis (2013) 18: 238. doi:10.1007/s10495-012-0788-0

Abstract

There is an increasing need to develop non-invasive molecular imaging strategies for visualizing and quantifying apoptosis status of diseases (especially for cancer) for diagnosis and monitoring treatment response. Since externalization of phosphatidylserine (PS) is one of the early molecular events during apoptosis, Annexin B1 (AnxB1), a member of Annexins family with high affinity toward the head group of PS, could be a potential positron emission tomography (PET) imaging probe for imaging cell death process after labeled by positron-emitting nuclides, such as 18F. In the present study, we investigated a novel PET probe, 18F-labeled Annexin B1 (18F-AnxB1), for apoptosis imaging. 18F-AnxB1 was prepared reliably by conjugating AnxB1 with a 18F-tag, N-succinimidyl 4-[18F]fluorobenzoate ([18F]SFB), in a radiolabeling yield of about 20 % within 40 min. The in vitro binding of 18F-AnxB1 with apoptotic cells induced by anti-Fas antibody showed twofold increase compared to those without treatment, confirmed by flow cytometric analysis with AnxV-FITC/PI staining. Stability tests demonstrated 18F-AnxB1 was rather stable in vitro and in vivo without degradation. The serial 18F-AnxB1 PET/CT scans in healthy rats outlined its biodistribution and pharmacokinetics, indicating a rapid renal clearance and predominant accumulation into kidney and bladder at 2 h p.i. 18F-AnxB1 PET/CT imaging was successfully applied to visualize in vivo apoptosis sites in tumor induced by chemotherapy and in kidney simulated by ischemia–reperfusion injury. The high-contrast images were obtained at 2 h p.i. to delineate apoptotic tumor. Apoptotic region could be still identified by 18F-AnxB1 PET 4 h p.i., despite the high probe retention in kidneys. In summary, we have developed 18F-AnxB1 as a PS-specific PET probe for the apoptosis detection and quantification which could have broad applications from disease diagnosis to treatment monitoring, especially in the cases of cancer.

Keywords

Apoptosis imaging18F-Annexin B118F-radiolabelingMolecular imaging probePET

Supplementary material

10495_2012_788_MOESM1_ESM.docx (2.7 mb)
Supplementary material 1 (DOCX 2,768 kb)

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Ming-Wei Wang
    • 1
    • 2
    • 3
  • Fang Wang
    • 4
  • Yu-Jia Zheng
    • 1
    • 2
    • 3
  • Ying-Jian Zhang
    • 1
    • 2
    • 3
  • Yong-Ping Zhang
    • 1
    • 2
    • 3
  • Qing Zhao
    • 1
    • 2
  • Clifton Kwang-Fu Shen
    • 5
    • 6
    • 7
  • Yue Wang
    • 4
  • Shu-Han Sun
    • 4
  1. 1.PET Center, Department of Nuclear MedicineFudan University Shanghai Cancer CenterShanghaiChina
  2. 2.Department of Oncology, Shanghai Medical CollegeFudan UniversityShanghaiChina
  3. 3.The Joint Center for Biomedical Imaging of Department of Chemistry & Institute of Biomedical Science & Shanghai Cancer CenterFudan UniversityShanghaiChina
  4. 4.Department of Medical GeneticsSecond Military Medical University (SMMU)ShanghaiChina
  5. 5.Department of Molecular and Medical PharmacologyDavid Geffen School of Medicine at University of California Los Angeles (UCLA)Los AngelesUSA
  6. 6.Crump Institute for Molecular Imaging (CIMI)David Geffen School of Medicine at University of California Los Angeles (UCLA)Los AngelesUSA
  7. 7.California NanoSystems Institute (CNSI)University of California Los Angeles (UCLA)Los AngelesUSA