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Rhodococcus psychrotolerans sp. nov., isolated from rhizosphere of Deschampsia antarctica

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Abstract

A novel actinobacterium, designated strain CMAA 1533T, was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533T was found to grow over a wide range of temperatures (4–28 °C) and pH (4–10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533T belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus. Sequence similarity calculations indicated that the novel strain is closely related to Rhodococcus degradans CCM 4446T, Rhodococcus erythropolis NBRC 15567T and Rhodococcus triatomae DSM 44892T (≤ 96.9%). The organism was found to contain meso-diaminopimelic acid, galactose and arabinose in whole cell hydrolysates. Its predominant isoprenologue was identified as MK-8(H2) and the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were identified as Summed feature (C16:1 ω6c and/or C16:1 ω7c), C16:0, C18:1 ω9c and 10-methyl C18:0. The G+C content of genomic DNA was determined to be 65.5 mol%. Unlike the closely related type strains, CMAA 1533T can grow at 4 °C but not at 37 °C and was able to utilise adonitol and galactose as sole carbon sources. Based on phylogenetic, chemotaxonomic and physiological data, it is concluded that strain CMAA 1533T (= NRRL B-65465T = DSM 104532T) represents a new species of the genus Rhodococcus, for which the name Rhodococcus psychrotolerans sp. nov. is proposed.

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Acknowledgements

Silva, L.J. thanks the support from National Council for Scientific and Technological Development [CNPq 141705/2014-0] and [CNPq PROANTAR 407230/2013-0, INCT Criosfera]. Genuario, D. B. and Souza, D.T were supported by FAPESP graduate scholarships 2014/26131-7 and 2013/25505-8, respectively. The authors are grateful to Marcia Maria Parma Leme, João Luiz da Silva, Renato Barbosa Salaroli and Roseli dos Santos Nascimento by their contributions with the laboratory techniques. Finally, thanks are due to the PROANTAR Research Program and Brazilian Navy for logistical support during the OPERANTAR EXPEDITION.

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Authors and Affiliations

  1. College of Agriculture “Luiz de Queiroz”, University of São Paulo, Pádua Dias Avenue, 11, Piracicaba, São Paulo, 13418-900, Brazil

    Leonardo Jose Silva & Harold Alexander Vargas Hoyos

  2. Laboratory of Environmental Microbiology, Brazilian Agricultural Research Corporation, EMBRAPA Environment, SP 340 Highway, Km 127.5, Jaguariúna, São Paulo, 13820-000, Brazil

    Leonardo Jose Silva, Danilo Tosta Souza, Diego Bonaldo Genuario, Harold Alexander Vargas Hoyos, Suikinai Nobre Santos & Itamar Soares Melo

  3. Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Presidente Antônio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    Luiz Henrique Rosa

  4. Agrivalle, Agricultural Biotechnology, Tranquillo Giannini Avenue, 1090, Salto, São Paulo, 13329-600, Brazil

    Tiago Domingues Zucchi

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  1. Leonardo Jose Silva
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  2. Danilo Tosta Souza
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Correspondence to Itamar Soares Melo.

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Silva, L.J., Souza, D.T., Genuario, D.B. et al. Rhodococcus psychrotolerans sp. nov., isolated from rhizosphere of Deschampsia antarctica . Antonie van Leeuwenhoek 111, 629–636 (2018). https://doi.org/10.1007/s10482-017-0983-7

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