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Characterization of Marinovum faecis sp. nov., an alphaproteobacterium isolated from marine sediment

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Abstract

A Gram-negative, strictly aerobic, chemoheterotrophic, beige-pigmented, ovoid bacterium, designated YP194T, was isolated from marine sediment in the Republic of Korea. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, with high sequence similarity (98.4%) to Marinovum algicola FF3T. The DNA–DNA relatedness values between strains YP194T and M. algicola FF3T were 34.1 ± 2.7%. The DNA G+C content of strain YP194T was 63.1 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acid was C18:1 ω7c (77.6%). Strain YP194T produced phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids as polar lipids. From the combination of genotypic and phenotypic characteristics and the distinct phylogenetic position, the strain is considered to represent a novel species of the genus Marinovum for which the name Marinovum faecis sp. nov. is proposed. The type strain of M. faecis sp. nov. is YP194T (= KCCM 90263T = NBRC 111905T).

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References

  • Atlas RM (2010) Handbook of microbiological media, 4th edn. CRC, Boca Raton

    Book  Google Scholar 

  • Beiss U (1964) Paper chromatographic separation of plant lipids. J Chromatogr 13:104–110

    Article  CAS  PubMed  Google Scholar 

  • Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    CAS  PubMed  Google Scholar 

  • Collins MD, Jones D (1981) A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51:129–134

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  PubMed  Google Scholar 

  • Fitch WM (1971) Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Garrity GM, Bell JA, Lilburn T (2005) Family I. Rhodobacteraceae fam. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2C, 2nd edn. Springer, New York, p 161

    Google Scholar 

  • Hansen GH, Sørheim R (1991) Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241

    Article  Google Scholar 

  • Iwaki H, Yasukawa N, Fujioka M, Takada K, Hasegawa Y (2013) Isolation and characterization of a marine cyclohexylacetate-degrading bacterium Lutimaribacter litoralis sp. nov., and reclassification of Oceanicola pacificus as Lutimaribacter pacificus comb. nov. Curr Microbiol 66:588–593

    Article  CAS  PubMed  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through the comparative studies of sequence evolution. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  • Lafay B, Ruimy R, de Traubenberg CR, Breittmayer V, Gauthier MJ, Christen R (1995) Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima. Int J Syst Bacteriol 45:290–296

    Article  CAS  PubMed  Google Scholar 

  • Lewin RA, Lounsbery DM (1969) Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58:145–170

    Article  CAS  PubMed  Google Scholar 

  • Martens T, Heidorn T, Pukall R, Simon M, Tindall BJ, Brinkhoff T et al (2006) Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56:1293–1304

    Article  CAS  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Park S, Park DS, Bae KS, Yoon JH (2014) Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64:1378–1383

    Article  CAS  PubMed  Google Scholar 

  • Power DA, Johnson JA (2009) Difco™ and BBL™ Manual: manual of microbiological culture media, 2nd edn. Becton Dickinson and Company, Sparks, pp 359–360

    Google Scholar 

  • Ruiz-Ponte C, Samain JF, Sanchez JL, Nicolas JL (1999) The benefit of a Roseobacter species on the survival of scallop larvae. Mar Biotechnol 1:52–59

    Article  CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newark

    Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Suzuki K, Kaneko T, Komagata K (1981) Deoxyribonucleic acid homologies among coryneform bacteria. Int J Syst Bacteriol 31:131–138

    Article  Google Scholar 

  • Tamura K, Peterson D, Petersen N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using Maximum Likelihood, evolutionary distance, and Maximum Parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Uchino Y, Hirata A, Yokota A, Sugiyama J (1998) Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44:201–210

    Article  CAS  PubMed  Google Scholar 

  • Wang L, Liu Y, Shi X, Wang Y, Zheng Y, Dai X, Zhang XH (2016) Xuhuaishuia manganoxidans gen. nov., sp. nov., amanganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province. Int J Syst Evol Microbiol 66:1521–1526

    Article  CAS  PubMed  Google Scholar 

  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Worliczek HL, Kämpfer P, Rosengarten R, Tindall RBJ, Busse HJ (2007) Polar lipid and fatty acid profiles-re-vitalizing old approaches as a modern tool for the classification of mycoplasmas? Syst Appl Microbiol 30:355–370

    Article  CAS  PubMed  Google Scholar 

  • Zhang G, Yang Y, Wang S, Sun Z, Jiao K (2015) Alkalimicrobium pacificum gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae. Int J Syst Evol Microbiol 65:2453–2458

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the National Research Foundation of Korea Grants (NRF-2014R1A1A2057302), Korea.

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Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. College of Pharmacy, Yeungnam University, 280 Daehak-ro, Gyeongsan, Gyeongbuk, 38541, Republic of Korea

    So Young Kim, Songhee Park & Hyukjae Choi

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  1. Jaewoo Yoon
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  2. So Young Kim
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  4. Hyukjae Choi
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Correspondence to Hyukjae Choi.

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10482_2017_867_MOESM1_ESM.pdf

Thin-layer chromatograms showing the total polar lipid compositions of YP194T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid, molybdenum blue, α-naphthol, ninhydrin and Dragendorff’s reagent. PG: phosphatidylglycerol, PE: phosphatidylenthanolamine, PC: phosphatidylcholine, UAL: unidentified aminolipid, UPL: unidentified phospholipid, UL: unidentified lipid Supplementary material 1 (PDF 57 kb)

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Yoon, J., Kim, S.Y., Park, S. et al. Characterization of Marinovum faecis sp. nov., an alphaproteobacterium isolated from marine sediment. Antonie van Leeuwenhoek 110, 963–969 (2017). https://doi.org/10.1007/s10482-017-0867-x

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  • DOI: https://doi.org/10.1007/s10482-017-0867-x

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