Abstract
Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: →4)-β-d-GlcpNAcA-(1 →3)-β-d-QuipNAc4NAc-(1 →3)-β-d-GalpNAc-(1 →. The 3D model, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 °C, this molecule displays only a low ice recrystallization inhibition activity.
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11 July 2017
An erratum to this article has been published.
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This work was supported by Grants from the Swedish Research Council and the Knut and Alice Wallenberg Foundation.
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An erratum to this article is available at https://doi.org/10.1007/s10482-017-0900-0.
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Casillo, A., Ståhle, J., Parrilli, E. et al. Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H. Antonie van Leeuwenhoek 110, 1377–1387 (2017). https://doi.org/10.1007/s10482-017-0834-6
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DOI: https://doi.org/10.1007/s10482-017-0834-6