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Enterovirga rhinocerotis gen. nov., sp. nov., isolated from Rhinoceros unicornis faeces

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Abstract

A novel strain, YIM 100770T, was isolated from Rhinoceros unicornis faeces collected from Yunnan Wild Animal Park, China. The taxonomic status was determined based on the physiological, biochemical and phylogenetic characteristics. Strain YIM 100770T was observed to be rod-shaped, non-motile, Gram-stain negative and aerobic. The G+C content of the genomic DNA was determined to be 68.5 mol%. The cells of strain YIM 100770T contain ubiquinone Q-10 as the respiratory quinone. The major fatty acids (>1%) were identified as Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 78.1%), Summed feature 4 (iso-C17:1-I and/or anteiso-C17:1-B; 12.9%), C19:0 cyclo ω8c (2.8%), C16:0 (2.2%) and C18:0 (2.2%). Comparison of 16S rRNA gene sequences revealed the strain show high similarities with the members of the genera Psychroglaciecola (94.5%), Methylobacterium (90.5–94.1%) and Microvirga (92.0–93.3%) in the family Methylobacteriaceae. In addition, the strain also showed high similarities with the members of the genera Chelatococcus (93.7–94.0%) and Pseudochelatococcus (93.1–93.7%) in the family Beijerinckiacea, and the genus Bosea (93.1–93.8%) in the family Bradyrhizobiaceae. The phylogenetic analysis, combined with the chemical characteristics, suggest that the strain represents a novel genus in the order Rhizobiales of the class Alphaproteobacteria, for which the name Enterovirga rhinocerotis gen. nov., sp. nov. is proposed. The type strain of E. rhinocerotis is YIM 100770T (=DSM 25903T = CCTCC AB 2012048T).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Nos. 31270001, 31460005 and 81573327), and supported by Program for New Century Excellent Talents in University and Yunnan Provincial Society Development Project (2014BC006). Thanks for the assistance of Yunnan Wild Animal Park, and thanks for the assistance from Mr. Jiang Liu and You-Long Li.

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    Authors and Affiliations

    1. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Xiu Chen, Qin-Yuan Li, Gui-Ding Li, Yi Jiang & Cheng-Lin Jiang

    2. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang, 110819, People’s Republic of China

      Xiu Chen, Gui-Ding Li, Hui Lei, Li Han & Xue-Shi Huang

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    1. Xiu Chen
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    Xiu Chen, Qin-Yuan Li contributed equally to this work.

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    Chen, X., Li, QY., Li, GD. et al. Enterovirga rhinocerotis gen. nov., sp. nov., isolated from Rhinoceros unicornis faeces. Antonie van Leeuwenhoek 110, 553–562 (2017). https://doi.org/10.1007/s10482-016-0823-1

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