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Rubricella aquisinus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae

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Abstract

A Gram-stain negative, ovoid or short rod-shaped, aerobic and non-motile bacterial strain, designated J82T, was isolated from a seawater sample collected from the coast of Yellow Sea in Qingdao, China. The strain grew at salinities of 1.0–6.0% (w/v) NaCl (optimum, 2.5%). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10–42 °C (optimum, 28–30 °C). The genomic DNA G + C content was determined to be 57.5 mol%. Q-10 was detected as the respiratory quinone. The major fatty acid (>10%) was Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and two unidentified polar lipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain J82T forms a distinct evolutionary lineage within the family Rhodobacteraceae. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the strain merits recognition as representative of a novel genus and species within the family Rhodobacteraceae for which the name Rubricella aquisinus gen. nov., sp. nov. is proposed. The type strain of Rubricella aquisinus is J82T (= DSM 103377T = CCTCC AB 2016170T).

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Acknowledgements

This work was supported by National Basic Research Program of China (2013CB955700), the National Key Research and Development Program of China (2016YFA0601402), the National Program on Global Change and Air-Sea Interaction (GASI-03-01-02-05), China Postdoctoral Science Foundation funded project (2016M592262), Key R&D projects in Shandong Province (2015GGF01014) and CNOOC Zhanjiang Branch (CNOOC-KJ 125 FZDXM 00 ZJ 001-2014). W-J Li was also supported by Project Supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People’s Republic of China

      Li-Qiang Yang, Lili Tang & Yongyu Zhang

    2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Lan Liu, Nimaichand Salam & Wen-Jun Li

    3. CNOOC China Limited, Zhanjiang Branch, Zhanjiang, 524057, People’s Republic of China

      Xiaowei Liu & Guangzhi Jin

    4. Institute of Marine Microbes and Ecospheres, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361101, People’s Republic of China

      Nianzhi Jiao

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    1. Li-Qiang Yang
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    2. Lili Tang
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    3. Lan Liu
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    Correspondence to Wen-Jun Li or Yongyu Zhang.

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    Li-Qiang Yang and Lili Tang have contributed equally to this work and share the first co-authorship.

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    Yang, LQ., Tang, L., Liu, L. et al. Rubricella aquisinus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae . Antonie van Leeuwenhoek 110, 331–337 (2017). https://doi.org/10.1007/s10482-016-0803-5

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