Abstract
The intrinsic ruggedness of Enterococcus faecalis is responsible for its widespread distribution in nature and is often viewed as an important virulence determinant. Previously, we showed that the ClpB ATPase is negatively regulated by CtsR and is required for thermotolerance and virulence in a Galleria mellonella invertebrate model. Here, we used in silico, Northern blot and quantitative real-time PCR analyses to identify additional members of the CtsR regulon, namely the clpP peptidase and the clpC and clpE ATPases. When compared to the parent strain, virulence of the ΔctsR strain in G. mellonella was significantly attenuated.
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Acknowledgments
This study was supported by Brazilian grants [Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ—Grant E-26/112.649/2012), Conselho Nacional do Desenvolvimento Científico e Tecnológico (CNPq—Grant 476119/2012-0), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES- Grant Proex 23038.001255/2011-29)] and by NIH/NIDCR award DE019783.
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Ana Paula Vaz Cassenego and Naira Elane Moreira de Oliveira have contributed equaly for the development of this work.
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Cassenego, A.P.V., de Oliveira, N.E.M., Laport, M.S. et al. The CtsR regulator controls the expression of clpC, clpE and clpP and is required for the virulence of Enterococcus faecalis in an invertebrate model. Antonie van Leeuwenhoek 109, 1253–1259 (2016). https://doi.org/10.1007/s10482-016-0727-0
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DOI: https://doi.org/10.1007/s10482-016-0727-0