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Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest

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Abstract

A novel Streptomyces strain, MUSC 119T, was isolated from a soil collected from a mangrove forest. Cells of MUSC 119T stained Gram-positive and formed light brownish grey aerial mycelium and grayish yellowish brown substrate mycelium on ISP 2 medium. A polyphasic approach was used to determine the taxonomic status of strain MUSC 119T, which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces. The cell wall peptidoglycan consisted of ll-diaminopimelic acid. The predominant menaquinones were identified as MK-9(H8), MK-9(H6) and MK-9(H4). The polar lipid profile consisted of phosphatidylinositol, phosphatidylethanolamine, glycolipids, diphosphatidylglycerol and four phospholipids. The predominant cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The cell wall sugars were glucose, mannose, ribose and rhamnose. The phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain MUSC119T to be closely related to Streptomyces rhizophilus JR-41T (99.0 % sequence similarity), S. panaciradicis 1MR-8T (98.9 %), S. gramineus JR-43T (98.8 %) and S. graminisoli JR-19T (98.7 %). These results suggest that MUSC 119T should be placed within the genus Streptomyces. DNA–DNA relatedness values between MUSC 119T to closely related strains ranged from 14.5 ± 1.3 to 27.5 ± 0.7 %. The G+C content was determined to be 72.6 mol  %. The polyphasic study of MUSC 119T showed that this strain represents a novel species, for which the name Streptomyces humi sp. nov. is proposed. The type strain of S. humi is MUSC 119T (=DSM 42174T = MCCC 1K00505T).

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Acknowledgments

This work was supported by the University of Malaya for High Impact Research Grant (UM-MOHE HIR Nature Microbiome Grant No. H-50001-A000027 and No. A000001-50001) awarded to K.-G. C. and External Industry Grant (Biotek Abadi-Vote No. GBA-808138), Fundamental Research Grant Scheme (FRGS/1/2013/SKK01/MUSM/03/3), MOSTI ScienceFund Grant (Project No. 06-02-10-SF0300) awarded to L.-H. L. Authors are grateful to Dr Jean Euzéby for the support in the Latin etymology of the new species name.

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Authors and Affiliations

  1. Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 46150, Bandar Sunway, Selangor Darul Ehsan, Malaysia

    Nurullhudda Zainal, Hooi-Leng Ser & Learn-Han Lee

  2. Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Nurullhudda Zainal, Wai-Fong Yin & Kok-Gan Chan

  3. Department of Medicine, Faculty of Medicine, Centre of Excellent for Research in AIDS (CERiA), University of Malaya, Kuala Lumpur, Malaysia

    Kok-Keng Tee

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  1. Nurullhudda Zainal
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Correspondence to Learn-Han Lee.

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Zainal, N., Ser, HL., Yin, WF. et al. Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest. Antonie van Leeuwenhoek 109, 467–474 (2016). https://doi.org/10.1007/s10482-016-0653-1

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