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Lentzea guizhouensis sp. nov., a novel lithophilous actinobacterium isolated from limestone from the Karst area, Guizhou, China

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Abstract

A novel filamentous actinobacterium, designated strain DHS C013T, was isolated from limestone collected in Guizhou Province, South-west China. Morphological and chemotaxonomic characteristics of the strain support its assignment to the genus Lentzea. Phylogenetic analyses showed that strain DHS C013T is closely related to Lentzea jiangxiensis FXJ1.034T (98.7 % 16S rRNA gene similarity) and Lentzea flaviverrucosa 4.0578T (98.0 % 16S rRNA gene similarity), but it can be distinguished from these strains based on low levels of DNA:DNA relatedness (~44 and ~37 %, respectively). Physiological and biochemical tests also allowed phenotypic differentiation of the novel strain from these closely related species. On the basis of the evidence presented here, strain DHS C013T is concluded to represent a novel species of the genus Lentzea, for which the name Lentzea guizhouensis sp. nov. is proposed. The type strain is DHS C013T (=KCTC 29677T = CGMCC 4.7203T).

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Acknowledgments

The authors are grateful to Prof. Ying Huang (State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.) for her kindly providing type strains. This study was jointly supported by the National Key Basic Research Program of China (No. 2013CB956702), the National Natural Science Foundation of China (No. 41373078), and the Natural Science Foundation of Jiangsu Normal University (No. 13XLA02).

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    Authors and Affiliations

    1. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou, People’s Republic of China

      Cheng-Liang Cao, Fa-Xiang Tao & Bin Lian

    2. University of Chinese Academy of Sciences, Beijing, People’s Republic of China

      Cheng-Liang Cao

    3. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, People’s Republic of China

      Cheng-Liang Cao, Xiao-Qi Zhou, Sheng Qin & Ji-Hong Jiang

    4. College of Life Science, Nanjing Normal University, Nanjing, Jiangsu, People’s Republic of China

      Bin Lian

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    1. Cheng-Liang Cao
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    Correspondence to Bin Lian.

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    Cheng-Liang Cao and Xiao-Qi Zhou have contributed equally to this work.

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    Cao, CL., Zhou, XQ., Qin, S. et al. Lentzea guizhouensis sp. nov., a novel lithophilous actinobacterium isolated from limestone from the Karst area, Guizhou, China. Antonie van Leeuwenhoek 108, 1365–1372 (2015). https://doi.org/10.1007/s10482-015-0589-x

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