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Occultifur kilbournensis f.a. sp. nov., a new member of the Cystobasidiales associated with maize (Zea mays) cultivation

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Abstract

During a study of microorganisms associated with maize (Zea mays) cultivation, yeasts were isolated from overwintered stalks, cobs and surrounding soil, which were collected from an agricultural field in south-central Illinois, USA. Predominant among isolates were two species of Cryptococcus (Cr. flavescens, Cr. magnus) and a red yeast that D1/D2 LSU rRNA gene sequences revealed to be a new species of the basidiomycete yeast genus Occultifur. The species, which was not detected in the same field during the growing season, is described here as Occultifur kilbournensis (MycoBank number MB 811259; type strain NRRL Y-63695, CBS 13982, GenBank numbers, D1/D2 LSU rRNA gene, KP413160, ITS, KP413162; allotype strain NRRL Y-63699, CBS 13983). Mixture of the type and allotype strains resulted in formation of hyphae with clamp connections and a small number of apparent basidia following incubation on 5 % malt extract agar at 15 °C for 2 months. In view of the uncertainty of the life cycle, the new species is being designated as forma asexualis. From analysis of D1/D2 and ITS nucleotide sequences, the new species is most closely related to Occultifur externus.

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Acknowledgments

We thank Andrey M. Yurkov, José Paulo Sampaio, Carlos A. Rosa and Teun Boekhout for comments on classification of the Cystobasidiales and for sharing unpublished data, Nathane Orwig for sequence determinations and Donald T. Wicklow and Jacob Brown for providing some of the field samples examined. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA

    Cletus P. Kurtzman & Christie J. Robnett

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  1. Cletus P. Kurtzman
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  2. Christie J. Robnett
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Correspondence to Cletus P. Kurtzman.

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10482_2015_427_MOESM1_ESM.docx

Fig. S1. Placement of Occultifur kilbournensis among members of the Cystobasidiales as determined from maximum likelihood analysis (HKY + G) of ITS nucleotide sequences. Rhodotorula glutinis and R. mucilaginosa were the designated outgroup species in the analysis. Bootstrap values (1000 replicates) >50 % are given at branch nodes. GenBank numbers follow strain numbers. Strain accession numbers: AS, Academia Sinica, Beijing, China; CBS, Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands; DMKU, S. Limtong, Thailand; IMUFRJ, Instituto de Microbiologia Prof. Paulo de Góe, Federal University of Rio de Janeiro; JCM, Japan Collection of Microorganisms, Tsukuba, Japan; NRRL, ARS Culture Collection, Peoria, Illinois, USA; UFMG =  Universidade Federal de Minas Gerais Culture Collection, Brazil. The newly described species Cystobasidium psychroaquaticum and C. rietchieii (Yurkov et al. 2015) were not included in the analysis. Type strains are indicated by a superscript T and superscript A indicates an authentic strain

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Kurtzman, C.P., Robnett, C.J. Occultifur kilbournensis f.a. sp. nov., a new member of the Cystobasidiales associated with maize (Zea mays) cultivation. Antonie van Leeuwenhoek 107, 1323–1329 (2015). https://doi.org/10.1007/s10482-015-0427-1

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  • DOI: https://doi.org/10.1007/s10482-015-0427-1

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