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Lysobacter lycopersici sp. nov., isolated from tomato plant Solanum lycopersicum

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Abstract

A Gram-stain negative, aerobic, rod-shaped and non-spore-forming bacterium, CC-Bw-6T was isolated from chopped tomato stems. Analysis of the 16S rRNA gene sequence of the strain CC-Bw-6T showed its affiliation with the genus Lysobacter within the class Gammaproteobacteria. Strain CC-Bw-6T was found to be most closely related to Lysobacter panaciterrae KCTC 12601T (97.0 %) and Lysobacter daecheongensis KCTC 12600T (96.8 %), and showed lower similarity (<96.5 %) to other Lysobacter species. DNA–DNA relatedness between strain CC-Bw-6T and L. panaciterrae KCTC 12601T was 10.8 %, the G+C content of the genomic DNA is 69.9 mol%. Strain CC-Bw-6T was determined to possess C11:0 iso, C11:0 iso 3OH, C14:0 iso, C15:0 anteiso, C15:1 ω5c, C16:1 ω5c, C16:0, C15:0 iso, C16:0 iso, C17:0 iso and C16:0 10-methyl/C17:1 iso ω9c as predominant fatty acids. The major polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids. The predominant polyamine is spermidine, and ubiquinone-8 is the predominant respiratory quinone. Analysis of phenotypic, geno- and phylogenetic characteristics revealed a distinct taxonomic position of strain CC-Bw-6T with respect to other Lysobacter species. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Lysobacter lycopersici sp. nov. The type strain is CC-Bw-6T (=BCRC 80612T = JCM 19164T).

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Acknowledgments

Authors would like to thank Professor Dr. Hans G. Trüper for his advice regarding the nomenclature. We also thank Dr. Mariyam Shahina for technical assistance. This research work was kindly supported by grants from the National Science Council, the Council of Agriculture, Executive Yuan and in part by the Ministry of Education, Taiwan, R.O.C. under the ATU plan.

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Authors and Affiliations

  1. Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 250, Kuo Kuang Rd., Taichung, 402, Taiwan

    Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Wei-An Lai & Chiu-Chung Young

  2. Bachelor Program of Bio-Industry Management, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan

    Cheng-Zhe Wen

  3. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

    Wei-An Lai & Chiu-Chung Young

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  1. Shih-Yao Lin
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Correspondence to Chiu-Chung Young.

Additional information

GenBank accession number for the 16S rRNA gene sequence of strain CC-Bw-6T is KC820654.

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Lin, SY., Hameed, A., Wen, CZ. et al. Lysobacter lycopersici sp. nov., isolated from tomato plant Solanum lycopersicum . Antonie van Leeuwenhoek 107, 1261–1270 (2015). https://doi.org/10.1007/s10482-015-0419-1

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