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Variovorax guangxiensis sp. nov., an aerobic, 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from banana rhizosphere

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Abstract

A 1-aminocyclopropane-1-carboxylate deaminase producing bacterium, designated GXGD002T, was isolated from the rhizosphere of banana plants cultivated in Guangxi province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXGD002T is a member of the genus Variovorax. High levels of 16S rRNA gene sequence similarity are found between strain GXGD002T and Variovorax paradoxus DSM 30034T (99.4 %), Variovorax ginsengisoli KCTC 12583T (99.1 %), Variovorax boronicumulans KCTC 22010T (99.0 %), Variovorax soli DSM18216T (98.7 %), Variovorax defluvii DSM 27259T (98.1 %) and Variovorax dokdonensis KCTC 12544T (97.4 %) respectively. However, the DNA–DNA hybridization values between strain GXGD002T and its closely related species V. paradoxus DSM 30034T, V. ginsengisoli KCTC 12583T and V. boronicumulans KCTC 22010T were found to be 40.7, 30.9 and 23.7 %, respectively. The DNA G + C content of strain GXGD002T was found to be 67.8 mol%. The major fatty acids of strain GXGD002T are C16:0 (20.3 %), C10:0 3OH (18.4 %), C17:0 cyclo (18.9 %), C18:1w7c (12.3 %) and summed feature 3 (13.9 %). The predominant respiratory quinone was identified as ubiquinone-8 (Q-8) and the major polar lipids as phosphatidylethanolamine and phosphatidylglycerol. The results of polyphasic taxonomic study including physiological and biochemical tests, whole-cell SDS-PAGE profiles and chemotaxonomic analysis allowed a clear differentiation of strain GXGD002T from the other species in the genus Variovorax. Based on these results, a new species, Variovorax guangxiensis, is proposed. The type strain is GXGD002T (=DSM 27352T = ACCC 05911T).

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Acknowledgments

This work was supported by “Special Fund for Agro-scientific Research in the Public Interest” (201203045) .The GenBank accession number for the 16S rRNA gene sequence of strain GXGD002T is JF495126.

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Authors and Affiliations

  1. Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097, People’s Republic of China

    Jun-lian Gao, Xu-ming Wang, Tian-lei Qiu, Ji-wei Li, Xiu-ai Li & Jian Chen

  2. Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Mei Yuan & Jian-guang Sun

  3. China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Hong-can Liu

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  1. Jun-lian Gao
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  2. Mei Yuan
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Correspondence to Jian-guang Sun.

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Gao, Jl., Yuan, M., Wang, Xm. et al. Variovorax guangxiensis sp. nov., an aerobic, 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from banana rhizosphere. Antonie van Leeuwenhoek 107, 65–72 (2015). https://doi.org/10.1007/s10482-014-0304-3

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