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Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater

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Abstract

A Gram-stain negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated GYSW-15T, was isolated from a seawater of Geoje island on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain GYSW-15T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GYSW-15T belongs to the genus Polaribacter, clustering coherently with the type strain of Polaribacter porphyrae showing sequence similarity value of 97.0 %. Strain GYSW-15T exhibited 16S rRNA gene sequence similarity values of 94.3–96.4 % to the type strains of the other Polaribacter species. Strain GYSW-15T was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, iso-C16:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The polar lipid profile of strain GYSW-15T containing phosphatidylethanolamine and an unidentified lipid as major components was similar to that of the type strain of P. porphyrae. The DNA G + C content of strain GYSW-15T was determined to be 31.2 mol% and its mean DNA–DNA relatedness with P. porphyrae LMG 26671T was 17 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GYSW-15T is separated from recognized Polaribacter species. On the basis of the data presented, strain GYSW-15T is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter marinivivus sp. nov. is proposed. The type strain is GYSW-15T ( = KCTC 42156T = CECT 8655T)

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Acknowledgments

This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources (grant NRF-2013M3A9A5075953) from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Ji-Min Park, Yong-Taek Jung & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Daejeon, Yuseong, South Korea

    Yong-Taek Jung

  3. Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Daejeon, Yuseong, South Korea

    Keun-Chul Lee & Jung-Sook Lee

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  1. Sooyeon Park
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  2. Ji-Min Park
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Correspondence to Jung-Hoon Yoon.

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Park, S., Park, JM., Jung, YT. et al. Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Antonie van Leeuwenhoek 106, 1139–1146 (2014). https://doi.org/10.1007/s10482-014-0283-4

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