Abstract
A Gram-negative, rod shaped, motile, aerobic bacterium, designated as strain AK49T was isolated from a water sample from a mangrove forest in Coringa village, Andhra Pradesh, India. Strain AK49T was observed to form yellow coloured, smooth, circular, convex colonies on marine agar, with entire margins. Cells of strain AK49T are 0.5–1.0 µm wide and 1.5–3.5 µm long. Growth was observed at 25–37 °C (optimum 30 °C), 2–6 % NaCl (optimum 2 %) and pH 6–8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain AK49T is closely related to two species recently reclassified as members of the genus Aliiglaciecola: Aliiglaciecola lipolytica JCM 15139T (sequence similarity 95.43 %) and Aliiglaciecola litoralis JCM 15896T (sequence similarity 96.91 %). The major cellular fatty acids of strain AK49T were found to include C16:0, C18:1ω7c and summed feature 3 (C16:1ω7c/C15:0 iso-2-OH). The polar lipid content of cell membrane was found to include phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified lipid and an unidentified glycolipid. The genomic DNA G+C content of strain AK49T was determined to be 41.9 mol%. Based on the taxonomic methods, including chemotaxonomic, phenotypic and phylogenetic approaches, strain AK49T is described here as a novel species belonging to the genus Aliiglaciecola, for which the name Aliiglaciecola coringensis sp. nov. is proposed. The type strain of Aliiglaciecola coringensis sp. nov. is AK49T (=MTCC 12003T = JCM19197T).
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Acknowledgments
We thank the Council of Scientific and Industrial Research (CSIR), the Directors of IMTECH, Chandigarh and NIO, Goa and Department of Biotechnology, as well as the Government of India for financial assistance and encouragement. We would like to thank Mr. Deepak for his excellent help in sequencing DNA. TNRS is thankful to project PSC0105 for providing facilities and funding, respectively. IMTECH communication number is 036/2014 and NIO contribution number is 5649.
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Gupta, V., Sharma, G., Srinivas, T.N.R. et al. Aliiglaciecola coringensis sp. nov., isolated from a water sample collected from mangrove forest in Coringa, Andhra Pradesh, India. Antonie van Leeuwenhoek 106, 1097–1103 (2014). https://doi.org/10.1007/s10482-014-0279-0
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DOI: https://doi.org/10.1007/s10482-014-0279-0