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Agarivorans litoreus sp. nov., a novel gammaproteobacterium isolated from seawater and emended description of the genus Agarivorans

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Abstract

A Gram-stain negative, aerobic, non-spore-forming, motile and rod-shaped or ovoid bacterial strain, designated GJSW-6T, was isolated from a seawater collected at Geoje island on the South Sea, South Korea, and subjected to polyphasic taxonomic study. Strain GJSW-6T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJSW-6T clustered with the type strains of two Agarivorans species, Agarivorans albus and Agarivorans gilvus, with which it exhibited sequence similarity values of 99.17 and 95.88 %, respectively. Sequence similarities to the type strains of the other recognized species were less than 93.04 %. Strain GJSW-6T was found to contain Q-8 as the predominant ubiquinone and C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C18:1 ω7c as the major fatty acids. The polar lipid profile of strain GJSW-6T containing phosphatidylethanolamine and phosphatidylglycerol as major components was similar to those of the type strains of A. albus and A. gilvus. The DNA G+C content of strain GJSW-6T was determined to be 45.3 mol% and its mean DNA–DNA relatedness value with A. albus JCM 21469T was 19 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-6T is separated from two Agarivorans species. On the basis of the data presented, strain GJSW-6T is considered to represent a novel species of the genus Agarivorans, for which the name Agarivorans litoreus sp. nov. is proposed. The type strain is GJSW-6T (= KCTC 42116T = NBRC 110444T). An emended description of the genus Agarivorans is also proposed.

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References

  • Barrow GI, Feltham RKA (1993) Cowan and steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Baumann P, Baumann L (1981) The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The Prokaryotes. Springer, Berlin, pp 1302–1331

    Google Scholar 

  • Bowman JP, McMeekin TA (2005) Order X. Alteromonadales ord. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, 2nd edn, vol. 2, part B. Springer, New York, p 443

  • Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006

    Article  PubMed  CAS  Google Scholar 

  • Cohen-Bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68

    Article  CAS  Google Scholar 

  • Du ZJ, Lv GQ, Rooney AP, Miao TT, Xu QQ, Chen GJ (2011) Agarivorans gilvus sp. nov. isolated from seaweed. Int J Syst Evol Microbiol 61:493–496

    Article  PubMed  CAS  Google Scholar 

  • Embley TM, Wait R (1994) Structural lipids of eubacteria. In: Goodfellow M, O’Donnell AG (eds) Modern Microbial Method: Chemical Methods in Prokaryotic Systematics. Wiley, Chichester, pp 121–161

    Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Komagata K, Suzuki KI (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  • Kurahashi M, Yokota A (2004) Agarivorans albus gen. nov., sp. nov., a & #x03B3;-proteobacterium isolated from marine animals. Int J Syst Evol Microbiol 54:693–697

    Article  PubMed  CAS  Google Scholar 

  • Lányí B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67

    Article  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Park S, Jung YT, Yoon JH (2013) Algibacter miyuki sp. nov., a member of the family Flavobacteriaceae isolated from leachate of a brown algae reservoir. Antonie Van Leeuwenhoek 104:253–260

    Article  PubMed  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. Microbial ID, Inc., Newark

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Staley JT (1968) Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942

    PubMed  CAS  PubMed Central  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, 9 other authors (1987). International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

  • Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY, Lee ST, Goodfellow M, Park YH (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505

    Article  CAS  Google Scholar 

  • Yoon JH, Lee ST, Park YH (1998) Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194

    Article  PubMed  CAS  Google Scholar 

  • Yoon JH, Kang KH, Park YH (2003) Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454

    Article  PubMed  CAS  Google Scholar 

  • Yoon JH, Kang SJ, Lee SY (2012) Salinimonas lutimaris sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat. Antonie Van Leeuwenhoek 101:803–810

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources (grant NRF-2013M3A9A5075953) from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-Gu, Suwon, South Korea

    Sooyeon Park, Ji-Min Park, Yong-Taek Jung & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea

    Yong-Taek Jung

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  1. Sooyeon Park
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  2. Ji-Min Park
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  3. Yong-Taek Jung
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  4. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

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Park, S., Park, JM., Jung, YT. et al. Agarivorans litoreus sp. nov., a novel gammaproteobacterium isolated from seawater and emended description of the genus Agarivorans . Antonie van Leeuwenhoek 106, 1041–1047 (2014). https://doi.org/10.1007/s10482-014-0273-6

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