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Roseivivax jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea

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Abstract

A Gram-stain negative, coccoid- or oval-shaped and non-gliding bacterial strain, designated CDM-17T, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea, and subjected to a polyphasic taxonomic study. Strain CDM-17T was observed to form smooth, circular, glistening, slightly convex, light yellowish pink colonies on marine agar, and was found to grow optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain CDM-17T fell within the clade comprising the Roseivivax species, clustering with the type strain of Roseivivax sediminis with which it exhibited 98.3 % sequence similarity value. Sequence similarities to the type strains of the other recognized Roseivivax species were 94.7–96.8 %. Strain CDM-17T was found to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) as the major fatty acid. The major polar lipids were identified as phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. The DNA G+C content of strain CDM-17T was determined to be 66.2 mol% and its mean DNA–DNA relatedness value with Rsv. sediminis KCTC 23444T was 17.5 ± 2.7 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain CDM-17T is distinguishable from recognized Roseivivax species. On the basis of the data presented, strain CDM-17T is proposed to represent a novel species of the genus Roseivivax, for which the name Roseivivax jejudonensis sp. nov. is proposed. The type strain is CDM-17T (=KCTC 42110T = CECT 8625T).

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Acknowledgments

This work was supported by a Grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK 21 Program from the Ministry of Science, ICT and Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Yong-Taek Jung & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Taejon, South Korea

    Yong-Taek Jung

  3. Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Taejon, South Korea

    Jung-Sook Lee

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  1. Yong-Taek Jung
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Correspondence to Jung-Hoon Yoon.

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Jung, YT., Lee, JS. & Yoon, JH. Roseivivax jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea. Antonie van Leeuwenhoek 106, 959–967 (2014). https://doi.org/10.1007/s10482-014-0265-6

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