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Thioclava atlantica sp. nov., isolated from deep sea sediment of the Atlantic Ocean

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Abstract

A taxonomic study was carried out on strain 13D2W-2T, which was isolated from a sulphur-oxidizing bacterial consortium, enriched by the deep-sea sediment of the Atlantic Ocean. The isolate was observed to be Gram-negative, oxidase- and catalase-positive, short rod-shaped and motile by means of a flagellum. Growth was observed at salinities from 0.5 to 12 % and at temperatures from 4 to 41 °C, and the strain found to be able to reduce nitrate but not degrade gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 13D2W-2T belongs to the genus Thioclava, with highest sequence similarity of 97.8 % to Thioclava dalianensis DLFJ1-1T, followed by Thioclava pacifica TL 2T (97.7 %), while the sequence similarities to other members of the genus were all below 97.0 %. The digital DNA:DNA hybridization estimated values between strain 13D2W-2T and, respectively, T. dalianensis DLFJ1-1T and T. pacifica TL 2T were 22.6 ± 2.4 and 25.6 ± 2.4 %. The ANI values between strain 13D2W-2T and T. dalianensis DLFJ1-1T and T. pacifica TL 2T were 78.49 and 81.91 % respectively. The principal fatty acid identified was Summed Feature 8 (C18:1 ω7c/ω6c) (74.38 %). The isoprenoid quinone of strain 13D2W-2T was identified as Q10 (100 %). The major polar lipids of strain 13D2W-2T were found to be comprised of phosphatidylethanolamine, phosphatidylglycerol, an aminophospholipid, a glycolipid and three unknown phospholipids. The G+C content of the chromosomal DNA was determined to be 65.3 mol%. The combined genotypic and phenotypic data show that strain 13D2W-2T represents a novel species of the genus Thioclava, for which the name Thioclava atlantica sp. nov. is proposed, with the type strain 13D2W-2T ( = MCCC 1A02612T = LMG 27145T).

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Abbreviations

MCCC:

Marine culture collection of China

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Acknowledgments

This work was financially supported by National Program on Key Basic Research Project (973 Program) (No.2012CB417300), COMRA Program (No. DY125-15-R-01), Public Welfare Project of SOA (201005032) and National Infrastructure of Natural Resources for Science and Technology Program of China (Nos. NIMR-2013-9 and NIMR-2014-9).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography SOA, Key Laboratory of Marine Genetic Resources of Fujian Province, Collaborative Innovation Center of Deep Sea Biology, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China

    Qiliang Lai, Shaoneng Li, Hongxiu Xu, Lijiang Jiang, Rongqiu Zhang & Zongze Shao

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  1. Qiliang Lai
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  2. Shaoneng Li
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  3. Hongxiu Xu
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  4. Lijiang Jiang
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  5. Rongqiu Zhang
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  6. Zongze Shao
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Corresponding author

Correspondence to Zongze Shao.

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Shaoneng Li and Qiliang Lai contributed equally to the paper.

Electronic supplementary material

Below is the link to the electronic supplementary material.

10482_2014_261_MOESM1_ESM.docx

Transmission electron micrographs of cells of strain 13D2W-2T and polar lipids of strain 13D2W-2T are available as supplementary figures in ANTO Online. (DOC 365 kb)

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Lai, Q., Li, S., Xu, H. et al. Thioclava atlantica sp. nov., isolated from deep sea sediment of the Atlantic Ocean. Antonie van Leeuwenhoek 106, 919–925 (2014). https://doi.org/10.1007/s10482-014-0261-x

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  • DOI: https://doi.org/10.1007/s10482-014-0261-x

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