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Clostridium vulturis sp. nov., isolated from the intestine of the cinereous vulture (Aegypius monachus)

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Abstract

A Gram-stain positive, strict anaerobe, spore-forming, motile rod-shaped bacterial strain with peritrichous flagella, designated YMB-57T, was isolated from the intestine of a cinereous vulture (Aegypius monachus) in Korea. StrainYMB-57T was found to show optimal growth at 37 °C, pH 7.5 and 1.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YMB-57T belongs to the genus Clostridium and is most closely related to the type strains of Clostridium subterminale (96.9 % sequence similarity), Clostridium thiosulfatireducens (96.7 %) and Clostridium sulfidigenes (96.6 %). The main fermentation end-products identified following growth in PYG medium were acetate, butyrate, ethanol, propanol, carbon dioxide and hydrogen. Peptone was converted to ethanol, and butanol, whereas glucose was fermented to ethanol. The major cellular fatty acids were identified as C16:0, C18:1 ω9c, and C18:1 ω9c DMA and the DNA G+C content was determined to be 34.0 mol%. Phenotypic and phylogenetic differences indicate that strain YMB-57T is distinct from other Clostridium species. It is proposed that strain YMB-57T be classified as the type strain of a novel species of the genus Clostridium, with the name Clostridium vulturis sp. nov. The type strain is YMB-57T (=KCTC 15114T = JCM 17998T).

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Acknowledgments

We are grateful to Dr. Jean. P. Euzeby (Society for Systematic and Veterinary Bacteriology, France) for his advice on nomenclature. This work was supported by grants RBM4611211, NMM0101232, and TGM0721311, and the KRIBB Research Initiative Program funded by the Ministry of Science, ICT & Future Planning, Republic of Korea. The authors would like to acknowledge funding from Korea Ministry of Environment as “Converging Technology Project (202–101–006)”.

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Authors and Affiliations

  1. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Jayoung Paek, Tae-Eun Jin, Yeseul Shin, In-Soon Park & Young-Hyo Chang

  2. Fermentation and Functionality Research Group, Korea Food Research Institute (KFRI), Sungnam, 463-746, Republic of Korea

    Mi-Hwa Lee

  3. The Research Institute of Industrial Science, Hanyang University, 17 Hangdang-dong, Seongdong-ku, Seoul, 133-791, Republic of Korea

    Byung-Chun Kim

  4. Department of Chemical Engineering, Department of Fuel Cells and Hydrogen Technology, Hanyang University, 17 Hangdang-dong, Seongdong-Ku, Seoul, 133-791, Republic of Korea

    Byoung-In Sang

  5. National Science Museum, 32-2 Guseong-dong, Yuseong-gu, Daejeon, 305-705, Republic of Korea

    Woon Kee Paek

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  1. Jayoung Paek
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Correspondence to Young-Hyo Chang.

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Jayoung Paek and Mi-Hwa Lee are contributed equally to this work.

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Paek, J., Lee, MH., Kim, BC. et al. Clostridium vulturis sp. nov., isolated from the intestine of the cinereous vulture (Aegypius monachus). Antonie van Leeuwenhoek 106, 577–583 (2014). https://doi.org/10.1007/s10482-014-0229-x

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