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Vibrio crosai sp. nov., isolated from a cultured oyster Crassostrea gigas

Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A motile, facultative anaerobic, marine bacterial isolate (CAIM 1437T) was obtained from a cultured oyster (Crassostrea gigas) in Sonora, México. The strain was studied by a phylogenetic analysis based on sequences of the 16S rRNA and five housekeeping genes, i.e. ftsZ, gapA, pyrH, recA, and topA. Comparison of the almost-complete 16S rRNA gene sequence with those of other type strains of the genus Vibrio showed a close relationship with the type strains of Vibrio orientalis and Vibrio rotiferianus, with similarity values ranging from 98.4 to 98.3 %, respectively. MLSA placed this strain within the Orientalis clade. The DNA–DNA hybridization value of strain CAIM 1437T with V. orientalis was 59 % and with V. rotiferianus 55 %. The DNA G+C content was determined to be 45.6 mol %. Phenotypic characteristics also showed differences with the species analysed. The results presented here support the description of a novel species, for which the name Vibrio crosai sp. nov. is proposed, with CAIM 1437T (= DSM 27145T) as the type strain.

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Abbreviations

MLSA:

Multilocus sequence analysis

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Acknowledgments

This work was partially funded by CONACYT project CB-2009-01 132328 and grant AGL-2010-18438 from the Ministerio de Ciencia e Innovación (Spain). Thanks to Carmen Bolán for technical support.

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Correspondence to Bruno Gomez-Gil.

Additional information

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, ftsZ, gapA, pyrH, recA, and topA gene sequences of strain CAIM 1437T are JQ434120, KC774631, KC774632, KC774630, KC774629 and KC774628, respectively; all other gene sequences used in this study are listed in the Supplementary Table 1.

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González-Castillo, A., Balboa, S., Romalde, J.L. et al. Vibrio crosai sp. nov., isolated from a cultured oyster Crassostrea gigas . Antonie van Leeuwenhoek 106, 457–463 (2014). https://doi.org/10.1007/s10482-014-0214-4

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  • DOI: https://doi.org/10.1007/s10482-014-0214-4

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