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Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran

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An Erratum to this article was published on 08 October 2013

Abstract

A Gram-negative, non-endospore-forming, rod shaped, strictly aerobic, moderately halophilic bacterium, designated strain M9BT, was isolated from the hypersaline lake Aran-Bidgol in Iran. Cells of strain M9BT were found to be motile and produce colonies with an orange-yellow pigment. Growth was determined to occur between 5 and 20 % (w/v) NaCl and the isolate grew optimally at 7.5–10 % (v/w) NaCl. The optimum pH and temperature for growth of the strain were determined to be pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of 6–8 and 25–45 °C, respectively. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain M9BT is a member of the genus Marinobacter. The closest relative to this strain was found to be Marinobacter hydrocarbonoclasticus MBIC 1303T with a similarity level of 97.7 %. DNA–DNA hybridization between the novel isolate and this phylogenetically related species was 13 ± 2 %. The major cellular fatty acids of the isolate were identified as C16:0, C19:1 ω6c, C18:1 ω9c and C16:1 ω9c. The polar lipid pattern of strain M9BT was determined to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and three phospholipids. Ubiquinone 9 (Q-9) was the only lipoquinone detected. The G+C content of the genomic DNA of this strain was determined to be 58.6 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus Marinobacter, for which the name Marinobacter persicus sp. nov. is proposed. The type strain of Marinobacter persicus is strain M9BT (=IBRC-M 10445T = CCM 7970T = CECT 7991T = KCTC 23561T).

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Acknowledgments

This work was supported by grants from Iranian Biological Resource Centre (IBRC) (MI-1388-04) (to M.A. Amoozegar), from Spanish Ministerio de Economía y Competitividad (CGL2010-19303) that include European Funds (FEDER), National Science Foundation (Grant DEB-0919290) and Junta de Andalucía (P10-CVI-6226) (to A. Ventosa).

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Authors and Affiliations

  1. Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, P. O. Box 14155-6455, Tehran, Iran

    Maryam Bagheri, Mohammad Ali Amoozegar & Maryam Didari

  2. Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran

    Maryam Bagheri & Mohammad Ali Amoozegar

  3. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Ali Makhdoumi-Kakhki

  4. DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Peter Schumann & Cathrin Spröer

  5. Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012, Sevilla, Spain

    Cristina Sánchez-Porro & Antonio Ventosa

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  1. Maryam Bagheri
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  2. Mohammad Ali Amoozegar
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  3. Maryam Didari
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  4. Ali Makhdoumi-Kakhki
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  5. Peter Schumann
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  7. Cristina Sánchez-Porro
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  8. Antonio Ventosa
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Correspondence to Mohammad Ali Amoozegar.

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Bagheri, M., Amoozegar, M.A., Didari, M. et al. Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran. Antonie van Leeuwenhoek 104, 47–54 (2013). https://doi.org/10.1007/s10482-013-9923-3

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