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Oceanospirillum nioense sp. nov., a marine bacterium isolated from sediment sample of Palk bay, India

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Abstract

A novel Gram-negative, spiral shaped, motile bacterium, designated strain NIO-S6T, was isolated from a sediment sample collected from Off-shore Rameswaram, Tamilnadu, India. Strain NIO-S6T was found to be positive for oxidase, DNase and lysine decarboxylase activities and negative for catalase, gelatinase, lipase, ornithine decarboxylase, nitrate reductase, aesculinase, amylase and urease activities. The fatty acids were determined to be dominated by C10:0 3OH, C16:0, C16:1 and C18:1. Strain NIO-S6T contains Q-8 as the major respiratory quinone. The DNA G+C content of the strain NIO-S6T was determined to be 49.5 ± 0.6 mol %. Phylogenetic analysis based on 16S rRNA gene sequence of strain NIO-S6T indicated Oceanospirillum linum and Oceanospirillum maris of the family Oceanospirillaceae (phylum Proteobacteria) are the closest related species with sequence similarities of 98.4 and 97.8 % respectively. Other members of the family showed sequence similarities <96.4 %. However, DNA–DNA hybridization with Oceanospirillum linum LMG 5214T and Oceanospirillum maris LMG 5213T showed a relatedness of 31.5 and 46.9 % with respect to strain NIO-S6T. Based on the phenotypic characteristics and on phylogenetic inference, strain NIO-S6T is proposed as a novel species of the genus Oceanospirillum as Oceanospirillum nioense sp. nov. and the type strain is NIO-S6T (=MTCC 11154T = KCTC 32008T).

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Acknowledgments

We thank Dr. J. Euzeby for his expert suggestion for the correct species epithet and Latin etymology. We also thank Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology, Government of India for financial assistance. We would like to thank Mr. Deepak for his help in 16S rRNA gene sequencing. The laboratory facility was extended by MMRF of NIO, RC, Kochi funded by the Ministry of Earth Sciences, New Delhi. TNRS is thankful to CSIR projects (SIP1302, SIP1308, OLP1201) other projects (GAP2131, SSP2490) for providing sample, facilities and funding. NIO contribution number is 5302.

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Authors and Affiliations

  1. CSIR-National Institute of Oceanography, Regional Centre, P B No. 1913, Dr. Salim Ali Road, Kochi, 682018, Kerala, India

    Kripa K. Krishna, Monson Thomas & T. N. R. Srinivas

  2. Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160 036, India

    V. Bhumika & P. Anil Kumar

Authors
  1. Kripa K. Krishna
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  2. V. Bhumika
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  3. Monson Thomas
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  4. P. Anil Kumar
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  5. T. N. R. Srinivas
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Corresponding author

Correspondence to T. N. R. Srinivas.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NIO-S6T is JN205304.

All authors contributed equally to the work.

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Krishna, K.K., Bhumika, V., Thomas, M. et al. Oceanospirillum nioense sp. nov., a marine bacterium isolated from sediment sample of Palk bay, India. Antonie van Leeuwenhoek 103, 1015–1021 (2013). https://doi.org/10.1007/s10482-013-9881-9

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