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Alteromonas australica sp. nov., isolated from the Tasman Sea

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Abstract

A non-pigmented, motile, Gram-negative bacterium designated H 17T was isolated from a seawater sample collected in Port Phillip Bay (the Tasman Sea, Pacific Ocean). The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17T was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C16:0, C16:1ω7, C17:1ω8 and C18:1ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17T belongs to the genus Alteromonas. The DNA−DNA relatedness between strain H 17T and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica, is proposed. The type strain is H 17T (= KMM 6016T = CIP 109921T).

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Acknowledgments

This study was partially supported by funds from Australian Research Council (ARC), by FEB RAS grant “Study of biological diversity of marine heterotrophic bacteria,” and by RFBR Grant No. 11-04-00781-a. The authors are grateful for financial support from the Ministry of Education and Science of the Russian Federation (Grant No. 14.B37.21.1231). The authors would like to thank Bio21 Institute for access to the MALDI-TOF MS instrument.

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Correspondence to Elena P. Ivanova.

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Ivanova, E.P., Ng, H.J., Webb, H.K. et al. Alteromonas australica sp. nov., isolated from the Tasman Sea. Antonie van Leeuwenhoek 103, 877–884 (2013). https://doi.org/10.1007/s10482-012-9869-x

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