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Thalassococcus lentus sp. nov., an alphaproteobacterium isolated from seawater of a seaweed farm

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Abstract

A Gram-negative, non-motile and rod- or ovoid-shaped bacterial strain, designated YCS-24T, was isolated from seawater of a seaweed farm in the South Sea, South Korea. Strain YCS-24T grew optimally at 25–28 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain YCS-24T exhibited the highest 16S rRNA gene sequence similarity values of 97.5 and 97.1 % to the type strains of Thalassobius maritimus and Thalassococcus halodurans, respectively. The neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain YCS-24T clustered with the type strain of T. halodurans. The DNA G+C content of strain YCS-24T was 58.0 mol% and its DNA–DNA relatedness values with T. halodurans JCM 13833T and T. maritimus GSW-M6T were 17 ± 6.2 and 23 ± 9.2 %, respectively. The predominant ubiquinone found in strain YCS-24T was Q-10 and the predominant fatty acid of strain YCS-24T was C18:1 ω7c. The major polar lipids of strain YCS-24T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain YCS-24T is distinguished from T. halodurans. On the basis of the data presented, strain YCS-24T (= KCTC 32084T = CCUG 62791T) represents a novel species of the genus Thalassococcus, for which the name Thalassococcus lentus sp. nov. is proposed.

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Acknowledgments

This study was supported by the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR) and BK 21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Soo-In Kim & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea

    Yong-Taek Jung

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  1. Sooyeon Park
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  2. Yong-Taek Jung
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  4. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YCS-24T is JX090308.

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Park, S., Jung, YT., Kim, SI. et al. Thalassococcus lentus sp. nov., an alphaproteobacterium isolated from seawater of a seaweed farm. Antonie van Leeuwenhoek 103, 465–473 (2013). https://doi.org/10.1007/s10482-012-9826-8

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  • DOI: https://doi.org/10.1007/s10482-012-9826-8

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