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Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China

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Abstract

A novel actinomycete, designated strain KLBMP 1262T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262T is most closely related to Amycolatopsis sulphurea DSM 46092T (97.96 %), Amycolatopsis ultiminotia RP-AC36T (97.50 %) and Amycolatopsis jejuensis N7-3T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C16:0, iso-C16:0 and iso-C15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262T (=KCTC 19885 T = NBRC 108679T).

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Acknowledgments

The authors are grateful to Prof. Iain C. Sutcliffe for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB180008, 11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Natural Science Foundation by Xuzhou City (No. XZZD1004).

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Authors and Affiliations

  1. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, People’s Republic of China

    Ke Xing, Yue-Ji Zhang, Guang-Kai Bian, Wen-Di Zhang, Sheng Qin & Ji-Hong Jiang

  2. Department of Pediatrics, Anyang Children’s Hospital, Anyang, 455000, People’s Republic of China

    Wei Liu

  3. NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Tomohiko Tamura

  4. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Jung-Sook Lee

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  1. Ke Xing
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  2. Wei Liu
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  3. Yue-Ji Zhang
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  4. Guang-Kai Bian
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  5. Wen-Di Zhang
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  6. Tomohiko Tamura
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  7. Jung-Sook Lee
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  8. Sheng Qin
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  9. Ji-Hong Jiang
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Corresponding authors

Correspondence to Sheng Qin or Ji-Hong Jiang.

Additional information

Ke Xing and Wei Liu contributed equally to this study.

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Xing, K., Liu, W., Zhang, YJ. et al. Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China. Antonie van Leeuwenhoek 103, 433–439 (2013). https://doi.org/10.1007/s10482-012-9823-y

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  • DOI: https://doi.org/10.1007/s10482-012-9823-y

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