Abstract
The Bacillus cereus group sensu lato includes six closely-related bacterial species: Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides and Bacillus weihenstephanensis. B. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. B. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16S rRNA gene and cspA genes. A total of seven housekeeping genes (glpF, gmK, ilvD, pta, purH, pycA and tpi) from different B. thuringiensis serovars and B. weihenstephanensis strains were amplified and their nucleotide sequences determined. A maximum likelihood phylogenetic tree was inferred from comparisons of the concatenated sequences. B. thuringiensis serovars navarrensis, bolivia and vazensis clustered not with the other B. thuringiensis serovars but rather with the B. weihenstephanensis strains, indicative of a common phylogeny. In addition, specific signature sequences and single nucleotide polymorphisms common to B. thuringiensis serovars navarrensis, bolivia and vazensis and the B. weihenstephanensis strains, and absent in the other B. thuringiensis serovars, were identified.
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References
Bartoszewicz M, Bideshi DK, Kraszewska A, Modzelewska E, Swiecicka I (2009) Natural isolates of Bacillus thuringiensis display genetic and psychrotrophic properties characteristic of Bacillus weihenstephanensis. J Appl Microbiol 106:1967–1975
Ferrandis MD, Andrew R, Porcar M, Iriarte J, Cosmao-Dumanoir V, Lecadet M-M, Caballero P, Ferré J (1999) Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys. Lett Appl Microbiol 28:440–444
Gevers D, Cohan FM, Lawrence JG, Spratt BG, Coenye T, Feil EJ, Stackebrandt E, Van de Peer Y, Vandamme P, Thompson FL, Swings J (2005) Re-evaluating prokaryotic species. Nat Rev Microbiol 3:733–739
Glare TR, O’Callaghan M (2000) Bacillus thuringiensis: biology, ecology and safety. Wiley, Toronto
González JM Jr, Dulmage HT, Carlton BC (1981) Correlation between specific plasmid and δ-endotoxin production in Bacillus thuringiensis. Plasmid 5:351–365
González JM Jr, Brown BJ, Carlton BC (1982) Transfer of Bacillus thuringiensis plasmids coding for δ-endotoxin among strains of B. thuringiensis and B. cereus. Proc Natl Acad Sci USA 79:6951–6955
Helgason E, Caugant DA, Lecadet MM, Chen Y, Mahillon J, Lövgren A, Hegna I, Kvaløy K, Kolstø A-B (1998) Genetic diversity of Bacillus cereus/B. thuringiensis isolates from natural sources. Curr Microbiol 37:80–87
Helgason E, Økstad OA, Caugant DA, Johansen HA, Fouet A, Mock M, Hegna I, Kolstø A-B (2000) Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—one species on the basis of genetic evidence. Appl Environ Microbiol 66:2627–263
Helgason E, Tourasse NJ, Meisal R, Caugant DA, Kolstø A-B (2004) Multilocus sequence typing scheme for bacteria of the Bacillus cereus group. Appl Environ Microbiol 70:191–201
Hill KK, Ticknor LO, Okinaka RT, Asay M, Blair H, Bliss KA, Laker M, Pardington PE, Richardson AP, Tonks M, Beecher DJ, Kemp JD, Kolstø A-B, Lee Wong AC, Keim P, Jackson PJ (2004) Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Appl Environ Microbiol 70:1068–1080
Iriarte J, Bel Y, Ferrandis MD, Andrew R, Murillo J, Ferré J, Caballero P (1998) Environmental distribution and diversity of Bacillus thuringiensis in Spain. System Appl Microbiol 21:97–106
Iriarte J, Cosmao Dumanoir V, Bel Y, Porcar M, Ferrandis MD, Lecadet M-M, Ferré J, Caballero P (2000) Characterization of Bacillus thuringiensis ser. balearica (serotype H48) and ser. navarrensis (serotype H50): Two novel serovars isolated in Spain. Curr Microbiol 40:17–22
Lecadet M-M, Frachon E, Cosmao Dumanoir V, Ripouteau H, Hamon S, Laurent P, Thiéry I (1999) Updating the H-antigen classification of Bacillus thuringiensis. J Appl Microbiol 86:660–672
Lechner S, Mayr R, Francis KP, Prüß BM, Kaplan T, Wießner-Gunkel E, Stewart GSAB, Scherer S (1998) Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. Int J Syst Bacteriol 48:1373–1382
Logan NA, De Vos P (2009) Genus I. Bacillus Cohn 1872, 174 dans. In: De Vos P, Guarrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer K-H, Whitman WB (eds) Bergey’s manual of systematic bacteriology, vol 3, 2nd edn. Springer, New York, pp 21–128
Priest FG, Barker M, Baillie LWJ, Holmes EC, Maiden MCJ (2004) Population structure and evolution of the Bacillus cereus group. J Bacteriol 186:7959–7970
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean HD (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62:775–806
Shisa N, Wasano N, Ohgushi A, Lee D-H, Ohba M (2002) Extremely high frequency of common flagellar antigens between Bacillus thuringiensis and Bacillus cereus. FEMS Microbiol Lett 213:93–96
Soufiane B, Côté J-C (2009) Discrimination among Bacillus thuringiensis H serotypes, serovars and strains based on 16S rRNA, gyrB and aroE gene sequence analyses. Antonie Van Leeuwenhoek 95:33–45
Soufiane B, Côté J-C (2010) Bacillus thuringiensis serovars bolivia, vazensis and navarrensis meet the description of Bacillus weihenstephanensis. Curr Microbiol 60:343–349
Soufiane B, Sirois M, Côté J-C (2011) Mutually exclusive distribution of the sap and eag S-layer genes and the lytB/lytA cell wall hydrolase genes in Bacillus thuringiensis. Antonie Van Leeuwenhoek 100:349–364
Stenfors LP, Mayr R, Scherer S, Granum PE (2002) Pathogenic potential of fifty Bacillus weihenstephanensis strains. FEMS Microbiol Lett 215:47–51
Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Bio Evol 10:512–526
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular Evolutionary Genetics Analysis using likelihood, distance, and parsimony methods. Mol Biol Evol 28:2731–2739
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Thorsen L, Hansen BM, Nielsen KF, Hendriksen NB, Phipps RK, Budde BB (2006) Characterization of emetic Bacillus weihenstephanensis, a new cereulide-producing bacterium. Appl Environ Microbiol 72:5118–5121
Tourasse NJ, Kolstø A-B (2008) SuperCAT: a supertree database for combined and integrative multilocus sequence typing analysis of the Bacillus cereus group of bacteria (including B. cereus, B. anthracis and B. thuringiensis). Nucleic Acids Res 36:D461–D468
Vilas-Bôas GT, Peruca AP, Arantes OM (2007) Biology and taxonomy of Bacillus cereus, Bacillus anthracis, and Bacillus thuringiensis. Can J Microbiol 53:673–687
von Stetten F, Mayr R, Scherer S (1999) Climatic influence on mesophilic Bacillus cereus and psychrotolerant Bacillus weihenstephanensis populations in tropical, temperate and alpine soil. Environ Microbiol 1:503–515
Xu D, Côté J-C (2006) Sequence diversity of the Bacillus thuringiensis, and B. cereus sensu lato flagellin (H-antigen) protein: comparison with H serotype diversity. Appl Environ Microbiol 72:4653–4662
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Soufiane, B., Baizet, M. & Côté, JC. Multilocus sequence analysis of Bacillus thuringiensis serovars navarrensis, bolivia and vazensis and Bacillus weihenstephanensis reveals a common phylogeny. Antonie van Leeuwenhoek 103, 195–205 (2013). https://doi.org/10.1007/s10482-012-9800-5
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DOI: https://doi.org/10.1007/s10482-012-9800-5