Skip to main content
SpringerLink
Log in

Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A novel filamentous bacterium, designated YIM 77831T, was isolated from a geothermal soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth occurred from 28 to 65 °C (optimum 50 °C), pH 6.0–8.0 (optimum pH 7.0). The strain formed branched substrate mycelia, endospores were produced on the substrate mycelium and aerial mycelium was not produced on any of the growth media tested. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM 77831T was affiliated with the family Thermoactinomycetaceae. The stain YIM 77831T contained meso-diaminopimelic acid in the cell wall. Whole-cell hydrolysates contained glucose, galactose, mannose, ribose and rhamnose. The polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid and four unknown phospholipids. The only menaquinone was MK-7. Major fatty acids were iso-C15:0, anteiso-C15:0 and anteiso-C17:0. The G+C content was 55.6 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77831T represents a novel genus and species, Lihuaxuella thermophila gen. nov., sp. nov., in the family Thermoactinomycetaceae. The type strain is YIM 77831T (CCTCC AA 2011024T = JCM 18059T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Addou AN, Schumann P, Sproer C, Hacene H, Cayol JL, Fardeau ML (2012) Melghirimyces algeriensis gen. nov., sp. nov., a member of the family Thermoactinomycetaceae, isolated from a salt lake. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.028985-0

    Google Scholar 

  • Cerny G (1978) Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. Appl Microbiol Biotechnol 5:113–122

    Article  CAS  Google Scholar 

  • Chen JJ, Lin LB, Zhang LL, Zhang J, Tang SK, Wei YL, Li WJ (2011) Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring. Syst Evol Microbiol 62:38–42

    Article  Google Scholar 

  • Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diaminobutyric acid. Appl Bacteriol 48:459–470

    Article  CAS  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    Article  PubMed  CAS  Google Scholar 

  • Dong XZ, Cai MY (2001) Manual of systematics and identification of general bacteria. Science Press, Beijing

    Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (2002) PHYLIP (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Science, University of Washington, Seattle

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov., an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715

    Article  PubMed  CAS  Google Scholar 

  • Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Microbiol 29:319–322

    Article  CAS  Google Scholar 

  • Hatayama K, Shoun H, Ueda Y, Nakamura A (2005) Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family ‘Thermoactinomycetaceae’ isolated from compost. Int J Syst Evol Microbiol 55:2101–2104

    Article  PubMed  CAS  Google Scholar 

  • Kelly KL (1964) Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. US Government Printing Office, Washington

    Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.038075-0

    Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2387

    Article  CAS  Google Scholar 

  • Lacey J, Cross T (1989) Genus Thermoactinomyces Tsiklinsky 1899, 501AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2574–2585

    Google Scholar 

  • Lechevalier MP, Lechevalier H (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443

    Article  CAS  Google Scholar 

  • Lechevalier MP, De Bièvre C, Lechevalier HA (1977) Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260

    Article  CAS  Google Scholar 

  • Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  PubMed  Google Scholar 

  • Li J, Zhang GT, Tian XP, Wang FZ, Zhang CS, Zhang S, Li WJ (2012) Marininema mesophilum gen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae. Int J Syst Evol Microbiol 62:1383–1388

    Article  PubMed  CAS  Google Scholar 

  • Locci R (1989) Streptomyces and related genera. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2451–2508

    Google Scholar 

  • Matsuo Y, Katsuta A, Matsuda S, Shizuri Y, Yokota A, Kasai H (2006) Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov. Int J Syst Evol Microbiol 56:2837–2842

    Article  PubMed  CAS  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    Article  CAS  Google Scholar 

  • Park DJ, Dastager SG, Lee JC, Yeo SH, Yoon JH, Kim CJ (2007) Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae. Int J Syst Evol Microbiol 57:2660–2664

    Article  PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Stackebrandt E, Woese CR (1981) Towards a phylogeny of the actinomycetes and related organisms. Curr Microbiol 5:197–202

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ (2009) Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59:2025–2032

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Tsilinsky P (1899) On the thermophilic moulds. Ann Inst Pasteur 13:500–505 (in French)

    Google Scholar 

  • Von Jan M, Riegger N, Pötter G, Schumann P, Verbarg S, Spröer C, Rohde M, Lauer B, Labeda DP, Klenk HP (2012) Kroppenstedtia eburnea gen. nov., sp. nov., a novel thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009. Int J Syst Evol Microbiol 61:2304–2310

    Article  Google Scholar 

  • Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2452–2492

    Google Scholar 

  • Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu H, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    Article  PubMed  CAS  Google Scholar 

  • Yassin AF, Hupfer H, Klenk HP, Siering C (2009) Desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family Thermoactinomycetaceae Matso et al. 2006. Int J Syst Evol Microbiol 59:454–459

    Article  PubMed  CAS  Google Scholar 

  • Yoon JH, Kim IG, Shin YK, Park YH (2005) Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 55:395–400

    Article  PubMed  CAS  Google Scholar 

  • Zhang YX, Dong C, Biao S (2007) Planifilum yunnanense sp. nov., a thermophilic thermoactinomycete isolated from a hot spring. Int J Syst Evol Microbiol 57:1851–1854

    Article  PubMed  Google Scholar 

  • Zhou ZH, Liu ZH, Qian YD, Kim SB, Goodfellow M (1998) Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Prof. J.P. Euzeby for his kind help to check and correct our Latin etymology for the genus and species name. This research was supported by National Natural Science Foundation of China (No. 31070007), International Cooperation Research Program of Yunnan Province (No. 2009AC017) and Yunnan Provincial Sciences and Technology Department (No. 2011FB034). W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

Author information

Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Tian-Tian Yu, Bing-Huo Zhang, Ji-Cheng Yao, Shu-Kun Tang, En-Min Zhou, Yi-Rui Yin & Wen-Jun Li

  2. College of Life Sciences, Jiujiang University, Jiujiang, 332000, People’s Republic of China

    Bing-Huo Zhang

  3. Faculty of Environmental Science and Engineering & Life Science and Technology, Kunming University of Science and Technology, Kunming, 650224, People’s Republic of China

    Da-Qiao Wei

  4. Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming

  5. Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Ürűmqi, 830011, People’s Republic of China

    Wen-Jun Li

Authors
  1. Tian-Tian Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bing-Huo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ji-Cheng Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shu-Kun Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. En-Min Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yi-Rui Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Da-Qiao Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hong Ming
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Wen-Jun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wen-Jun Li.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 462 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, TT., Zhang, BH., Yao, JC. et al. Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 102, 711–718 (2012). https://doi.org/10.1007/s10482-012-9771-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-012-9771-6

Keywords

Search

Navigation