CHRO RESEARCH

Antonie van Leeuwenhoek

, Volume 92, Issue 3, pp 319-330

Wolinella succinogenes response to ox-bile stress

  • Bernice M. TanAffiliated withSchool of Biotechnology and Biomolecular Sciences, The University of New South Wales
  • , Quoc V. TuAffiliated withSchool of Medical Sciences, The University of New South Wales
  • , Zsuzsanna KovachAffiliated withSchool of Medical Sciences, The University of New South Wales
  • , Mark RafteryAffiliated withBioanalytical Mass Spectrometry Facility, The University of New South Wales
  • , George L. MendzAffiliated withSchool of Medical Sciences, The University of New South Wales Email author 

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Abstract

The bacterium Wolinella succinogenes is the only known species of its genus. It was first isolated from cow ruminal fluid, and in cattle, it dwells in the reticulum and rumen compartments of the stomach. The global protein response of W. succinogenes to ox-bile was investigated with the aim to understand bile-tolerance mechanisms of the bacterium. Bacteria were grown in liquid media supplemented with different bile concentrations to determine its effects on growth and morphology. Proteomic analyses served to identify 14 proteins whose expression was modulated by the presence of 0.2% bile. Quantitative real-time PCR analyses of the expression of selected genes were employed to obtain independent confirmation of the proteomics data. Proteins differentially expressed revealed metabolic pathways involved in the adaptation of W. succinogenes to bile. The data suggested that bile stress elicited complex physiological responses rather than just specific pathways, and identified proteins previously unknown to be involved in the adaptation of bacteria to bile.

Keywords

Wolinella succinogenes Bile response 2D-PAGE Proteomics Quantitative RT-PCR Modulation of protein expression