Skip to main content

Advertisement

SpringerLink
Log in

ISSR and SSR markers reveal sex-specific DNA sequences in three Calamus species from India

  • Published:
Agroforestry Systems Aims and scope Submit manuscript

Abstract

Calamus species yields the raw materials for the cane industry. However, the extraction of the cane from the forests is being carried out indiscriminately without considering the sustenance of the species. Plants of both sexes should co-exist for reproductive success. The sex of Calamus plants can be identified only after flowering and hence proper planning of managed forestry practices cannot be accomplished. A study was carried out in this background and male specific ISSR markers for C. tenuis and C. flagellum and SSR markers for C. thwaitesii were identified. The diagnostic potential of these markers can be exploited to sex the Calamus species at the seedling stage for proper breeding and agroforestry management.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Banerjee NS, Manoj P, Das MR (1999) Male sex associated RAPD markers in Piper longum L. Curr Sci 77:693–695

    CAS  Google Scholar 

  • Basu SK (1992) Rattans (canes) in India, a monographic revision. Rattan Information Center, Kepong

    Google Scholar 

  • Belcher B, Rujehan, Imang N, Achdiawan R (2004) Rattan, rubber, or oil palm: cultural and financial considerations for farmers in kalimantan. Econ Bot 58(1):S77–S87. doi:10.1663/0013-0001(2004)58[77:RROOPC]2.0.CO;2

    Article  Google Scholar 

  • Bizard V (2013) Rattan futures in Katingan: why do smallholders abandon or keep their gardens in Indonesia’s ‘rattan district’? Working Paper 175. World Agroforestry Centre (ICRAF) Southeast Asia Regional Program, Bogor. doi: 10.5716/WP13251.PDF

  • Chaves-Bedoya G, Nunenz V (2007) A SCAR marker for the sex types determination in Colombian genotypes of Carica papaya. Euphytica 153:215–220

    Article  CAS  Google Scholar 

  • Danilova TV, Karlov GI (2006) Application of inter simple sequence repeat (ISSR) polymorphism for detection of sex-specific molecular markers in hop (Humulus lupulus L.). Euphytica 151:15–21

    Article  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hick JB (1983) A plant DNA mini preparation; Version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Eichenseer C, Will J, Rampf M, Wend S, Greil P (2010) Biomorphous porous hydroxyapatite-ceramics from rattan (Calamus rotang). J Mater Sci Mater Med 21:131–137

    Article  CAS  PubMed  Google Scholar 

  • Flachowsky H, Schuman E, Weber WE, Peil A (2001) Application of AFLP for detection of sex-specific markers in hemp. Plant Breed 120:305–309

    Article  CAS  Google Scholar 

  • Gangopadhyay G, Roy SK, Ghose K, Poddar R, Bandyopadhyay T, Basu D, Mukherjee KK (2007) Sex detection of Carica papaya and Cycas circinalis in pre-flowering stage by ISSR and RAPD. Curr Sci 92:524–526

    CAS  Google Scholar 

  • Garcia-Fernandez C, Casado MA (2005) Forest recovery in managed agroforestry systems: the case of benzoin and rattan gardens in Indonesia. For Ecol Manag 214(1–3):158–169

    Article  Google Scholar 

  • Godoy R (1990) The economics of traditional rattan cultivation. Agrofor Syst 12(2):163–172. doi:10.1007/BF00123471

    Article  Google Scholar 

  • Godoy RA, Feaw TC (1991) Agricultural diversification among smallholder rattan cultivators in Central Kalimantan, Indonesia. Agrofor Syst 13(1):27–40. doi:10.1007/BF00129617

    Article  Google Scholar 

  • Goel CL (1992) Tips on cultivation and harvesting techniques of canes (rattans) in India. In: Rattan management and utilization. Proceedings rattan-India seminar, pp. 174–179

  • Hormaza JL, Dollo L, Polito VS (1994) Identification of a RAPD marker linked to sex determination in Pistacia vera using bulk segregant analysis. Theor Appl Genet 89:9–13

    Article  CAS  PubMed  Google Scholar 

  • Inoue M, Lahjie AM (1990) Dynamics of swidden agriculture in East Kalimantan. Agrofor Syst 12(3):269–284. doi:10.1007/BF00137289

    Article  Google Scholar 

  • Michelmore R, Paran I, Keselli V (1991) Identification of markers linked to disease-resistance genes by bulk segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mohan RHY, Tandon R (1997) Bamboo and rattans: from riches to rags. Proc Indian Natl Sci Acad 63:245–267

    Google Scholar 

  • Nagaoka T, Ogihara Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597–602

    Article  CAS  Google Scholar 

  • Nghitoolwa E, Hall JB, Sinclair FL (2003) Population status and gender imbalance of the marula tree, Sclerocarya birrea subsp. caffra in northern Namibia. Agrofor Syst 59(3):289–294

    Article  Google Scholar 

  • Pandalai RC (2002) Underplanting rattans in rubber plantations (KFRI Research Report No.245). Peechi, Thrissur

  • Parasnis A, Ramakrishna W, Chowdari K, Grupta V, Ranjekar P (1999) Microsatellite (GATA)n reveals sex-specific differences in papaya. Theor Appl Genet 99:1047–1052

    Article  CAS  Google Scholar 

  • Prakash S, Van Staden J (2006) Sex identification in Encephalartos natalensis (Dyer and Verdoorn) using RAPD markers. Euphytica 152:197–200

    Article  CAS  Google Scholar 

  • Reamon-Buettner SM, Jung C (2000) AFLP-derived STS markers for the identification of sex in Asparagus officinalis L. Theor Appl Genet 100:432–438

    Article  Google Scholar 

  • Sarmah P, Sarma RN (2011) Identification of ISSR marker linked to sex determination in Calamus tenius Roxb. An economically important rattan species of Northeast India. Mol Breed 27:115–118

    Article  Google Scholar 

  • Siebert SF (2000) Survival and growth of rattan intercropped with coffee and cacao in the agroforests of Indonesia. Agrofor Syst 50(1):95–102. doi:10.1023/A:1006481902824

    Article  Google Scholar 

  • Tsumura Y, Ohba K, Strauss SH (1996) Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theor Appl Genet 92:40–45

    Article  CAS  PubMed  Google Scholar 

  • Urasaki N, Tokumoto M, Tarora K, Ban Y, Kayano T, Tanaka H, Oku H, Chinen I, Terauchi R (2002) A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.). Theor Appl Genet 104:281–285

    Article  CAS  PubMed  Google Scholar 

  • Williams JGK, Rubelik AR, Livak KJ, Rafalski A, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu WJ, Wang BW, Cui KM (2004) RAPD and SCAR markers linked to sex determination in Eucommia ulmoides Oliv. Euphytica 136:233–238

    Article  CAS  Google Scholar 

  • Yakubov B, Barazani O, Golan-Goldhirsh A (2005) Combination of SCAR primers and Touchdown-PCR for sex identification in Pistacia vera L. Sci Hortic 103:473–478

    Article  CAS  Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176–183

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thankfully acknowledge the financial help received from the Department of Biotechnology, Government of India in the form of DBT Twinning Project for NER, 2011-2014 (Order No. BT/156/NE/TBP/2011) for this work. We extend our sincere thanks to Director, JNTBGRI and Vice Chancellor, AAU for providing necessary facilities. We also extend our special thanks to, the Director, RFRI (ICFRE), Deovan, Jorhat, Assam and Kerala and Assam State Forest Departments for granting permission to collect leaf samples of the rattan species from various forest regions (Kolibor, Golaghat, Rongdoi, Sibsagar, Joypur forest reserve of Assam & Kerala Forest Department: Order No. WL 10-3053/2012). The authors express their gratitude to Mr Gunin Saikia, Forest Conservator, Jorhat District and Mr. Shefeek S. for assistance during plant collection trips.

Author information

Authors and Affiliations

  1. Assam Agricultural University, Jorhat, Assam, 785013, India

    Prabalee Sarmah, Vishwa J. Barua, Jyotismrita Nath & R. N. Sarma

  2. Jawaharlal Nehru Tropical Botanical Garden and Research Institute (JNTBGRI), Thiruvananthapuram, Kerala, 695562, India

    Binoy Kurian, A. S. Hemanthkumar & K. K. Sabu

Authors
  1. Prabalee Sarmah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vishwa J. Barua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jyotismrita Nath
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. N. Sarma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Binoy Kurian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. S. Hemanthkumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. K. Sabu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. K. Sabu.

Ethics declarations

Conflict of interest

Authors declare no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 64 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sarmah, P., Barua, V.J., Nath, J. et al. ISSR and SSR markers reveal sex-specific DNA sequences in three Calamus species from India. Agroforest Syst 91, 509–513 (2017). https://doi.org/10.1007/s10457-016-9952-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10457-016-9952-9

Keywords

Search

Navigation