Angiogenesis

, Volume 15, Issue 1, pp 33–45

Exosomes released by K562 chronic myeloid leukemia cells promote angiogenesis in a src-dependent fashion

  • Marco Mineo
  • Susan H. Garfield
  • Simona Taverna
  • Anna Flugy
  • Giacomo De Leo
  • Riccardo Alessandro
  • Elise C. Kohn
Original Paper

DOI: 10.1007/s10456-011-9241-1

Cite this article as:
Mineo, M., Garfield, S.H., Taverna, S. et al. Angiogenesis (2012) 15: 33. doi:10.1007/s10456-011-9241-1

Abstract

Exosomes, microvesicles of endocytic origin released by normal and tumor cells, play an important role in cell-to-cell communication. Angiogenesis has been shown to regulate progression of chronic myeloid leukemia (CML). The mechanism through which this happens has not been elucidated. We isolated and characterized exosomes from K562 CML cells and evaluated their effects on human umbilical endothelial cells (HUVECs). Fluorescent-labeled exosomes were internalized by HUVECs during tubular differentiation on Matrigel. Exosome localization was perinuclear early in differentiation, moving peripherally in cells undergoing elongation and connection. Exosomes move within and between nanotubular structures connecting the remodeling endothelial cells. They stimulated angiotube formation over a serum/growth factor-limited medium control, doubling total cumulative tube length (P = 0.003). Treatment of K562 cells with two clinically active tyrosine kinase inhibitors, imatinib and dasatinib, reduced their total exosome release (P < 0.009); equivalent concentrations of drug-treated exosomes induced a similar extent of tubular differentiation. However, dasatinib treatment of HUVECs markedly inhibited HUVEC response to drug control CML exosomes (P < 0.002). In an in vivo mouse Matrigel plug model angiogenesis was induced by K562 exosomes and abrogated by oral dasatinib treatment (P < 0.01). K562 exosomes induced dasatinib-sensitive Src phosphorylation and activation of downstream Src pathway proteins in HUVECs. Imatinib was minimally active against exosome stimulation of HUVEC cell differentiation and signaling. Thus, CML cell-derived exosomes induce angiogenic activity in HUVEC cells. The inhibitory effect of dasatinib on exosome production and vascular differentiation and signaling reveals a key role for Src in both the leukemia and its microenvironment.

Keywords

ExosomesNanotubesChronic myeloid leukemiaEndothelial cellsTyrosine kinase inhibitors

Supplementary material

10456_2011_9241_MOESM1_ESM.tif (1.1 mb)
K562 exosomes are preferentially taken up by endothelial cells. HUVECs (a, b) and CCD27sK fibroblasts (c, d) were plated on glass and incubated for 4 h with PKH26-labeled exosomes (red). After the incubation, cell were fixed and stained for nuclei (blue) and actin (green). Scale bars: 20 μm (TIFF 1096 kb)
View video

Transfer of PKH26-stained K562 exosomes through nanotubes connecting HUVECs. Exosomes were labeled with PKH26, incubated with HUVECs on Matrigel, and after 3 h, the cells were monitored by time-lapse video-microscopy. Movie 1 was acquired at 1 frame/15 s for a period of 5.5 min. Movie 2 was acquired at 1 frame/30 s for a period of 25 min (MPG 48634 kb)

View video

Transfer of PKH26-stained K562 exosomes through nanotubes connecting HUVECs. Exosomes were labeled with PKH26, incubated with HUVECs on Matrigel, and after 3 h, the cells were monitored by time-lapse video-microscopy. Movie 1 was acquired at 1 frame/15 s for a period of 5.5 min. Movie 2 was acquired at 1 frame/30 s for a period of 25 min (MPG 99193 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Marco Mineo
    • 1
    • 3
  • Susan H. Garfield
    • 2
  • Simona Taverna
    • 3
  • Anna Flugy
    • 3
  • Giacomo De Leo
    • 3
  • Riccardo Alessandro
    • 3
  • Elise C. Kohn
    • 1
  1. 1.Molecular Signaling Section, Medical Oncology Branch, Center for Cancer ResearchNational Cancer InstituteBethesdaUSA
  2. 2.CCR Confocal Microscopy Core Facility, Laboratory of Experimental Carcinogenesis, Center for Cancer ResearchNational Cancer InstituteBethesdaUSA
  3. 3.Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Sezione di Biologia e GeneticaUniversità di PalermoPalermoItaly